Characterization of novel transcripts of human papillomavirus type 16 using CAGE technology

Transcriptome analysis of HPV-infected cells using CAGE and 3′ RACE shows the presence of transcripts on the minus strand of the HPV genome, antisense to the known protein-coding genes.

Our research unit joined this collaboration by providing our protocol for 3′ RACE analysis of non-polyadenylated transcripts. We are now working on the characterisation of these transcripts more in details using high-throughput sequencing of full-length cDNAs.


Reference:

Taguchi A, Nagasaka K, Kawana K, Hashimoto K, Kusumoto-Matsuo R, Plessy C, Thomas M, Nakamura H, Bonetti A, Oda K, Kukimoto I, Carninci P, Banks L, Osuga Y, Fujii T. Characterization of novel transcripts of human papillomavirus type 16 using CAGE technology. J Virol. 2015 Feb;89(4):2448-52 PubMed: 25505068