Digital expression profiling of the compartmentalized translatome of Purkinje neurons
In collaboration with Molecular Neurocybernetics Unit at the the RIKEN's Brain Science Institute, we have profiled the translatome of Purkinje neurons, to identify expressed proteins and the non-coding RNAs that may regulate their translation. The press release on RIKEN's website summarises our findings.
In this work, we made an extensive use of the our CAGEscan approach to link promoters to functional transcripts. Since the study was done on rats, in which gene models are not comprehensive in 5′, CAGEscan was instrumental to distinguish main promoters of known genes from novel promoters of unknown genes. This article is the reference use for the CAGEscan clustering tools for which we released the source code in 2013.
Note added on August 6th: see also the analysis of hippocampal dendritic RNA using a similar method, published recently by Ainsley et al.
Kratz A☮, Beguin P☮, Kaneko M, Chimura T, Suzuki AM, Matsunaga A, Kato S, Bertin N, Lassmann T, Vigot R, Carninci P, Plessy C, Launey T. Digital expression profiling of the compartmentalized translatome of Purkinje neurons. Genome Research. 2014 Aug;24(8):1396-410 PubMed: 24904046