Template switching

Template switching (TS) is the method that we use to add linkers at the 5′ end of our nanoCAGE libraries.

Here is a quick bibliographic timeline (work in progress).

1996

  • Clontech patent from Alex Chenchik, York Zhu, Luda Diatchenko and Paul Siebert (Methods and compositions for full-length cDNA Cloning using a template-switching oligonucleotide, US 5962272 A).

1998

  • Book chapter from Chenchik et al., cited in other manuscripts, but unfortunately not available online.

1999

  • Matz et al. apply TS to RACE-PCR and introduce step-out PCR to reduce noise caused by the TS oligonucleotide being used as a reverse-transcription primer.

2001

  • Zhu et al., apply TS to the construction of full-length cDNA libraries.

Bibliography

Chenchik A., Zhu Y.Y., Diatchenko L., Li R., Hill J., Siebert P.D. Gene Cloning and Analysis by RT-PCR. Siebert P., Larrick J. , editors. Natick, MA: BioTechniques Books; 1998. p. 305-319.