Template switching (TS) is the method that we use to add linkers at the 5′ end of our nanoCAGE libraries.
Here is a quick bibliographic timeline (work in progress).
- Clontech patent from Alex Chenchik, York Zhu, Luda Diatchenko and Paul Siebert (Methods and compositions for full-length cDNA Cloning using a template-switching oligonucleotide, US 5962272 A).
- Book chapter from Chenchik et al., cited in other manuscripts, but unfortunately not available online.
- Matz et al. apply TS to RACE-PCR and introduce step-out PCR to reduce noise caused by the TS oligonucleotide being used as a reverse-transcription primer.
- Zhu et al., apply TS to the construction of full-length cDNA libraries.
Zhu YY1, Machleder EM, Chenchik A, Li R, Siebert PD. Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction. Biotechniques. 2001 Apr;30(4):892-7 PubMed: 11314272
Matz M1, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A. Amplification of cDNA ends based on template-switching effect and step-out PCR. Nucleic Acids Res. 1999 Mar 15;27(6):1558-60 PubMed: 10037822
Chenchik A., Zhu Y.Y., Diatchenko L., Li R., Hill J., Siebert P.D. Gene Cloning and Analysis by RT-PCR. Siebert P., Larrick J. , editors. Natick, MA: BioTechniques Books; 1998. p. 305-319.