Recent changes to this wiki:

updated PO files
diff --git a/Links.ja.po b/Links.ja.po
index e8c3299..818f594 100644
--- a/Links.ja.po
+++ b/Links.ja.po
@@ -2,7 +2,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: GMTU\n"
-"POT-Creation-Date: 2013-11-17 08:26+0000\n"
+"POT-Creation-Date: 2017-05-01 06:31+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: GMTU\n"
@@ -52,7 +52,8 @@ msgid ""
 "(http://www.debian.org/) operating system and in particular its Blend "
 "[Debian Med](http://www.debian.org/devel/debian-med).  The [Zenbu](http://"
 "fantom.gsc.riken.jp/zenbu) genome browser is particularly adapted for "
-"visualising CAGE libraries."
+"visualising CAGE libraries.  Find our source code on [GitHub](https://github."
+"com/Population-Transcriptomics)."
 msgstr ""
 
 #. type: Plain text

Link to GitHub page.
diff --git a/Links.mdwn b/Links.mdwn
index ba35345..b9b514c 100644
--- a/Links.mdwn
+++ b/Links.mdwn
@@ -33,7 +33,8 @@ These tools run on Unix systems, for instance the
 [Debian](http://www.debian.org/) operating system and in particular its Blend
 [Debian Med](http://www.debian.org/devel/debian-med).  The
 [Zenbu](http://fantom.gsc.riken.jp/zenbu) genome browser is particularly
-adapted for visualising CAGE libraries. 
+adapted for visualising CAGE libraries.  Find our source code on
+[GitHub](https://github.com/Population-Transcriptomics).
 
 We are supported by RIKEN and the Japanese Ministry Education, Culture, Sports,
 Science and Technology ([MEXT](http://www.mext.go.jp/english/)), the Japanese

Correct date.
diff --git a/bibliography/28349422.mdwn b/bibliography/28349422.mdwn
index 53653f7..8e9f267 100644
--- a/bibliography/28349422.mdwn
+++ b/bibliography/28349422.mdwn
@@ -1,5 +1,5 @@
 [[!tag bibliography nanoCAGE People/Charles_Plessy]]
-[[!meta date="01 Apr 2017"]]
+[[!meta date="28 Mar 2017"]]
 
 Poulain S, Kato S, Arnaud O, Morlighem JÉ, Suzuki M, Plessy C<sup>✉</sup>, Harbers M<sup>✉</sup>.
 NanoCAGE: A Method for the Analysis of Coding and Noncoding 5'-Capped Transcriptomes.

Remove spurious paragraphs.
diff --git a/bibliography/28349422.mdwn b/bibliography/28349422.mdwn
index 579d751..53653f7 100644
--- a/bibliography/28349422.mdwn
+++ b/bibliography/28349422.mdwn
@@ -2,8 +2,6 @@
 [[!meta date="01 Apr 2017"]]
 
 Poulain S, Kato S, Arnaud O, Morlighem JÉ, Suzuki M, Plessy C<sup>✉</sup>, Harbers M<sup>✉</sup>.
-
 NanoCAGE: A Method for the Analysis of Coding and Noncoding 5'-Capped Transcriptomes.
-
 [[!doi 10.1007/978-1-4939-6716-2_4 desc="Methods Mol Biol. **2017** 1543:57-109"]]
 [[!PMID 28349422]]

Book chapter published.
diff --git a/bibliography/28349422.mdwn b/bibliography/28349422.mdwn
new file mode 100644
index 0000000..579d751
--- /dev/null
+++ b/bibliography/28349422.mdwn
@@ -0,0 +1,9 @@
+[[!tag bibliography nanoCAGE People/Charles_Plessy]]
+[[!meta date="01 Apr 2017"]]
+
+Poulain S, Kato S, Arnaud O, Morlighem JÉ, Suzuki M, Plessy C<sup>✉</sup>, Harbers M<sup>✉</sup>.
+
+NanoCAGE: A Method for the Analysis of Coding and Noncoding 5'-Capped Transcriptomes.
+
+[[!doi 10.1007/978-1-4939-6716-2_4 desc="Methods Mol Biol. **2017** 1543:57-109"]]
+[[!PMID 28349422]]

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index 48a64fe..73abd47 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-08-25 06:29+0000\n"
+"POT-Creation-Date: 2017-01-25 01:01+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -251,6 +251,22 @@ msgid ""
 "polymerases, add non-templated As to blunt DNA duplexes."
 msgstr ""
 
+#. type: Plain text
+#, no-wrap
+msgid ""
+"Since the extra <em>G</em>s do not originate from the genome, make sure that\n"
+"your alignment pipeline can tolerate their mismatches.  Also, depending on how\n"
+"the mismatches are represented, make sur that they do not cause a shift in the\n"
+"TSS in your downstream analyses.  To process the alignment produced by the\n"
+"command `bwa sampe`, we added a new flag (`-extraG`) to the\n"
+"[pairedBamToBed12](ttps://github.com/Population-Transcriptomics/pairedBamToBed12)\n"
+"tool, to remove mismatched <em>G</em>s at 5′ ends.   (Of course, this will miss\n"
+"the case where the extra _G_ matches a _G_ on the genome by chance, but\n"
+"visually inspection of the results, comparing ENCODE HeLa libraries with\n"
+"nanoCAGE HeLa libraries, convinced us that this simple approach was already a\n"
+"considerable improvement over the non-corrected data.)\n"
+msgstr ""
+
 #. type: Title ###
 #, no-wrap
 msgid "<a name=\"CAGEscan\">What is the difference between _nanoCAGE_ and _CAGEscan_ ?</a>"

More on extra Gs.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index ca57b0e..c6a363e 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -138,6 +138,18 @@ _template-switching_ mechanism, nanoCAGE libraries are enriched in
    Jan;279(1):142-53](https://pubmed.gov/22035236), showith that reverse-transcriptases,
    like other DNA polymerases, add non-templated As to blunt DNA duplexes.
 
+Since the extra <em>G</em>s do not originate from the genome, make sure that
+your alignment pipeline can tolerate their mismatches.  Also, depending on how
+the mismatches are represented, make sur that they do not cause a shift in the
+TSS in your downstream analyses.  To process the alignment produced by the
+command `bwa sampe`, we added a new flag (`-extraG`) to the
+[pairedBamToBed12](ttps://github.com/Population-Transcriptomics/pairedBamToBed12)
+tool, to remove mismatched <em>G</em>s at 5′ ends.   (Of course, this will miss
+the case where the extra _G_ matches a _G_ on the genome by chance, but
+visually inspection of the results, comparing ENCODE HeLa libraries with
+nanoCAGE HeLa libraries, convinced us that this simple approach was already a
+considerable improvement over the non-corrected data.)
+
 ### <a name="CAGEscan">What is the difference between _nanoCAGE_ and _CAGEscan_ ?</a>
 
 _nanoCAGE_ is a CAGE protocol using _template switching_ to enrich for 5′ ends.

updated PO files
diff --git a/People/Charles_Plessy.ja.po b/People/Charles_Plessy.ja.po
index 7300c69..17111fd 100644
--- a/People/Charles_Plessy.ja.po
+++ b/People/Charles_Plessy.ja.po
@@ -1,7 +1,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2015-11-17 05:26+0000\n"
+"POT-Creation-Date: 2016-11-15 14:34+0000\n"
 "PO-Revision-Date: 2014-07-07 20:05+0900\n"
 "Last-Translator: Charles Plessy <plessy@riken.jp>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -38,18 +38,32 @@ msgstr ""
 "Expression](http://fantom.gsc.riken.jp/protocols/))の研究を行ってきました。"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "I have developed a miniaturized version of CAGE, termed [[nanoCAGE]], to "
+#| "analyse small samples yielding only nanograms of RNA ([Plessy et al., "
+#| "2010](http://pubmed.gov/20543846)).  In the same manuscript, we also "
+#| "introduced its paired-end variant, CAGEscan, which we use to associate "
+#| "novel promoters with annotations.  Since then, we have kept improving or "
+#| "expanding these techniques, by updating the protocol ([Salimullah et al., "
+#| "2011](http://pubmed.org/21205859)), reducing the sequence bias introduced "
+#| "by the molecular barcodes ([Tang et al., 2013](http://pubmed."
+#| "org/23180801)), combining multiple cap-enrichment steps ([Batut et al., "
+#| "2013](http://pubmed.org/22936248)), and benchmarking the use of locked "
+#| "nucleic acids for template switching ([Harbers et al., 2013](http://"
+#| "pubmed.org/24079827))."
 msgid ""
 "I have developed a miniaturized version of CAGE, termed [[nanoCAGE]], to "
 "analyse small samples yielding only nanograms of RNA ([Plessy et al., 2010]"
 "(http://pubmed.gov/20543846)).  In the same manuscript, we also introduced "
 "its paired-end variant, CAGEscan, which we use to associate novel promoters "
 "with annotations.  Since then, we have kept improving or expanding these "
-"techniques, by updating the protocol ([Salimullah et al., 2011](http://"
-"pubmed.org/21205859)), reducing the sequence bias introduced by the "
-"molecular barcodes ([Tang et al., 2013](http://pubmed.org/23180801)), "
-"combining multiple cap-enrichment steps ([Batut et al., 2013](http://pubmed."
-"org/22936248)), and benchmarking the use of locked nucleic acids for "
-"template switching ([Harbers et al., 2013](http://pubmed.org/24079827))."
+"techniques, by updating the protocol ([Salimullah et al., 2011](https://"
+"pubmed.gov/21205859)), reducing the sequence bias introduced by the "
+"molecular barcodes ([Tang et al., 2013](https://pubmed.gov/23180801)), "
+"combining multiple cap-enrichment steps ([Batut et al., 2013](https://pubmed."
+"gov/22936248)), and benchmarking the use of locked nucleic acids for "
+"template switching ([Harbers et al., 2013](https://pubmed.gov/24079827))."
 msgstr ""
 "そして、いわばCAGE法のミニチュア版であり、わずかナノグラム単位のRNAしか含まな"
 "い微量の試料を分析できる[[nanoCAGE]]法を開発 ([Plessy et al., 2010](http://"
@@ -63,13 +77,23 @@ msgstr ""
 "を行い、これらの技術の改良および拡充を続けています。"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "I have complemented my work on CAGE with the development of a gene-"
+#| "centred technique for detecting promoters, termed Deep-RACE ([Olivarius "
+#| "et al., 2009](https://www.ncbi.nlm.nih.gov/pubmed/19317658), [Plessy et "
+#| "al., 2012](http://dx.doi.org/10.1002/9783527644582.ch4)), which we used "
+#| "to validate our discovery of the pervasive expression of retrotransposons "
+#| "detected by CAGE ([Faulkner et al., 2009](http://pubmed.gov/19377475)).  "
+#| "With contributions related to CAGE development and analysis, I am member "
+#| "of the [FANTOM](http://fantom.gsc.riken.jp/) consortium since FANTOM3."
 msgid ""
 "I have complemented my work on CAGE with the development of a gene-centred "
 "technique for detecting promoters, termed Deep-RACE ([Olivarius et al., 2009]"
 "(https://www.ncbi.nlm.nih.gov/pubmed/19317658), [Plessy et al., 2012](http://"
 "dx.doi.org/10.1002/9783527644582.ch4)), which we used to validate our "
 "discovery of the pervasive expression of retrotransposons detected by CAGE "
-"([Faulkner et al., 2009](http://pubmed.gov/19377475)).  With contributions "
+"([Faulkner et al., 2009](https://pubmed.gov/19377475)).  With contributions "
 "related to CAGE development and analysis, I am member of the [FANTOM](http://"
 "fantom.gsc.riken.jp/) consortium since FANTOM3."
 msgstr ""
@@ -82,15 +106,27 @@ msgstr ""
 "FANTOMコンソーシアムのメンバーとしてもCAGE法の開発と分析に貢献しています。"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "Together with my colleagues at RIKEN and collaborators in the field of "
+#| "neuroscience, I have applied nanoCAGE to the study of single neuron cell "
+#| "types, for instance the olfactory neurons ([Plessy et al., 2012](http://"
+#| "pubmed.org/22194471)), or in dopaminergic cells, where we could "
+#| "demonstrate the expression of haemoglobin in the midbrain ([Biagioli et "
+#| "al., 2009](http://pubmed.org/19717439)).  We are also exploring the sub-"
+#| "cellular localisation of RNA in Purkinje neurons ([Kratz et al, 2014]"
+#| "(http://pubmed.org/24904046)).  In parallel with this promoter-centric "
+#| "work, I am also exploring the huge [repertoire](http://clonotyper."
+#| "branchable.com) of the T cell antigen receptors."
 msgid ""
 "Together with my colleagues at RIKEN and collaborators in the field of "
 "neuroscience, I have applied nanoCAGE to the study of single neuron cell "
-"types, for instance the olfactory neurons ([Plessy et al., 2012](http://"
-"pubmed.org/22194471)), or in dopaminergic cells, where we could demonstrate "
+"types, for instance the olfactory neurons ([Plessy et al., 2012](https://"
+"pubmed.gov/22194471)), or in dopaminergic cells, where we could demonstrate "
 "the expression of haemoglobin in the midbrain ([Biagioli et al., 2009]"
-"(http://pubmed.org/19717439)).  We are also exploring the sub-cellular "
-"localisation of RNA in Purkinje neurons ([Kratz et al, 2014](http://pubmed."
-"org/24904046)).  In parallel with this promoter-centric work, I am also "
+"(https://pubmed.gov/19717439)).  We are also exploring the sub-cellular "
+"localisation of RNA in Purkinje neurons ([Kratz et al, 2014](https://pubmed."
+"gov/24904046)).  In parallel with this promoter-centric work, I am also "
 "exploring the huge [repertoire](http://clonotyper.branchable.com) of the T "
 "cell antigen receptors."
 msgstr ""

https://pubmed.gov
diff --git a/People/Charles_Plessy.mdwn b/People/Charles_Plessy.mdwn
index 2148764..a61b8f5 100644
--- a/People/Charles_Plessy.mdwn
+++ b/People/Charles_Plessy.mdwn
@@ -16,29 +16,29 @@ to analyse small samples yielding only nanograms of RNA ([Plessy et al.,
 its paired-end variant, CAGEscan, which we use to associate novel promoters
 with annotations.  Since then, we have kept improving or expanding these
 techniques, by updating the protocol ([Salimullah et al.,
-2011](http://pubmed.org/21205859)), reducing the sequence bias introduced by
-the molecular barcodes ([Tang et al., 2013](http://pubmed.org/23180801)),
+2011](https://pubmed.gov/21205859)), reducing the sequence bias introduced by
+the molecular barcodes ([Tang et al., 2013](https://pubmed.gov/23180801)),
 combining multiple cap-enrichment steps ([Batut et al.,
-2013](http://pubmed.org/22936248)), and benchmarking the use of locked nucleic
+2013](https://pubmed.gov/22936248)), and benchmarking the use of locked nucleic
 acids for template switching  ([Harbers et al.,
-2013](http://pubmed.org/24079827)).
+2013](https://pubmed.gov/24079827)).
 
 I have complemented my work on CAGE with the development of a gene-centred
 technique for detecting promoters, termed Deep-RACE ([Olivarius et al.,
 2009](https://www.ncbi.nlm.nih.gov/pubmed/19317658), [Plessy et al.,
 2012](http://dx.doi.org/10.1002/9783527644582.ch4)), which we used to validate
 our discovery of the pervasive expression of retrotransposons detected by CAGE
-([Faulkner et al., 2009](http://pubmed.gov/19377475)).  With contributions
+([Faulkner et al., 2009](https://pubmed.gov/19377475)).  With contributions
 related to CAGE development and analysis, I am member of the
 [FANTOM](http://fantom.gsc.riken.jp/) consortium since FANTOM3.
 
 Together with my colleagues at RIKEN and collaborators in the field of
 neuroscience, I have applied nanoCAGE to the study of single neuron cell types,
 for instance the olfactory neurons ([Plessy et al.,
-2012](http://pubmed.org/22194471)), or in dopaminergic cells, where we could
+2012](https://pubmed.gov/22194471)), or in dopaminergic cells, where we could
 demonstrate the expression of haemoglobin in the midbrain ([Biagioli et al.,
-2009](http://pubmed.org/19717439)).  We are also exploring the sub-cellular
-localisation of RNA in Purkinje neurons ([Kratz et al, 2014](http://pubmed.org/24904046)).
+2009](https://pubmed.gov/19717439)).  We are also exploring the sub-cellular
+localisation of RNA in Purkinje neurons ([Kratz et al, 2014](https://pubmed.gov/24904046)).
 In parallel with this promoter-centric work, I am also exploring the huge
 [repertoire](http://clonotyper.branchable.com) of the T cell antigen receptors.
 

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index d2518b4..48a64fe 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-08-15 04:53+0000\n"
+"POT-Creation-Date: 2016-08-25 06:29+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -225,14 +225,6 @@ msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). _Reverse transcriptase "
-"template switching: a SMART approach for full-length cDNA library "
-"construction._ [BioTechniques 30, 892-897](https://pubmed.gov/11314272), "
-"showing that nevertheless template switching also occurs on blunt duplexes."
-msgstr ""
-
-#. type: Bullet: ' - '
-msgid ""
 "Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). _The human L1 "
 "promoter: variable transcription initiation sites and a major impact of "
 "upstream flanking sequence on promoter activity._ [Genome Res. 2004 "

I do not remember what I meant about the Zhu paper; therefore I cut that part.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 03f912d..ca57b0e 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -122,10 +122,6 @@ _template-switching_ mechanism, nanoCAGE libraries are enriched in
    nucleotide addition using an anchor ligation method._ [DNA Res. 11,
    305-309](https://pubmed.gov/15500255), showing that A-caps are
    reverse-transcribed as Ts.
- - Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). _Reverse transcriptase
-   template switching: a SMART approach for full-length cDNA library
-   construction._ [BioTechniques 30, 892-897](https://pubmed.gov/11314272), showing
-   that nevertheless template switching also occurs on blunt duplexes.
  - Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). _The human L1
    promoter: variable transcription initiation sites and a major impact of
    upstream flanking sequence on promoter activity._ [Genome Res. 2004

updated PO files
diff --git a/TemplateSwitching.ja.po b/TemplateSwitching.ja.po
new file mode 100644
index 0000000..c4af511
--- /dev/null
+++ b/TemplateSwitching.ja.po
@@ -0,0 +1,89 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2016-08-25 06:23+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Title =
+#, no-wrap
+msgid "Template switching\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"_Template switching_ (TS) is the method that we use to add linkers at the 5′ "
+"end of our [[nanoCAGE]] libraries."
+msgstr ""
+
+#. type: Plain text
+msgid "Here is a quick bibliographic timeline (work in progress)."
+msgstr ""
+
+#. type: Title ###
+#, no-wrap
+msgid "1996"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Clontech patent from Alex Chenchik, York Zhu, Luda Diatchenko and Paul "
+"Siebert (_Methods and compositions for full-length cDNA Cloning using a "
+"template-switching oligonucleotide_, [US 5962272 "
+"A](http://www.google.com/patents/US5962272))."
+msgstr ""
+
+#. type: Title ###
+#, no-wrap
+msgid "1998"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Book chapter from Chenchik _et al._, cited in other manuscripts, but "
+"unfortunately not available online."
+msgstr ""
+
+#. type: Title ###
+#, no-wrap
+msgid "1999"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Matz _et al._ apply TS to RACE-PCR and introduce _step-out PCR_ to reduce "
+"noise caused by the TS oligonucleotide being used as a reverse-transcription "
+"primer."
+msgstr ""
+
+#. type: Title ###
+#, no-wrap
+msgid "2001"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid "Zhu _et al._, apply TS to the construction of full-length cDNA libraries."
+msgstr ""
+
+#. type: Title -
+#, no-wrap
+msgid "Bibliography\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!inline pages=\"bibliography/* and tagged(template_switching)\" "
+"template=biblio feeds=no show=0]]\n"
+msgstr ""

Short bibliography on template switching.
diff --git a/TemplateSwitching.mdwn b/TemplateSwitching.mdwn
new file mode 100644
index 0000000..3d1c3a8
--- /dev/null
+++ b/TemplateSwitching.mdwn
@@ -0,0 +1,35 @@
+Template switching
+==================
+
+_Template switching_ (TS) is the method that we use to add linkers at the 5′
+end of our [[nanoCAGE]] libraries.
+
+Here is a quick bibliographic timeline (work in progress).
+
+### 1996
+
+ - Clontech patent from Alex Chenchik, York Zhu, Luda Diatchenko and Paul
+   Siebert (_Methods and compositions for full-length cDNA Cloning using a
+   template-switching oligonucleotide_, [US 5962272
+   A](http://www.google.com/patents/US5962272)).
+
+### 1998
+
+ - Book chapter from Chenchik _et al._, cited in other manuscripts, but
+   unfortunately not available online.
+
+### 1999
+
+ - Matz _et al._ apply TS to RACE-PCR and introduce _step-out PCR_ to reduce
+   noise caused by the TS oligonucleotide being used as a reverse-transcription
+   primer.   
+
+### 2001
+
+ - Zhu _et al._, apply TS to the construction of full-length cDNA libraries.
+
+
+Bibliography
+------------
+
+[[!inline pages="bibliography/* and tagged(template_switching)" template=biblio feeds=no show=0]]
diff --git a/bibliography/10037822.mdwn b/bibliography/10037822.mdwn
new file mode 100644
index 0000000..860c5b8
--- /dev/null
+++ b/bibliography/10037822.mdwn
@@ -0,0 +1,7 @@
+[[!tag bibliography template_switching]]
+[[!meta date="1 Mar 1999"]]
+
+Matz M1, Shagin D, Bogdanova E, Britanova O, Lukyanov S, Diatchenko L, Chenchik A.
+_Amplification of cDNA ends based on template-switching effect and step-out PCR._
+[[!doi 10.1093/nar/27.6.1558 desc="Nucleic Acids Res. **1999** Mar 15;27(6):1558-60"]]
+[[!PMID 10037822]]
diff --git a/bibliography/11314272.mdwn b/bibliography/11314272.mdwn
new file mode 100644
index 0000000..f46d0a2
--- /dev/null
+++ b/bibliography/11314272.mdwn
@@ -0,0 +1,7 @@
+[[!tag bibliography template_switching]]
+[[!meta date="1 Apr 2001"]]
+
+Zhu YY1, Machleder EM, Chenchik A, Li R, Siebert PD.
+_Reverse transcriptase template switching: a SMART approach for full-length cDNA library construction._
+[Biotechniques. **2001** Apr;30(4):892-7](http://www.biotechniques.com/multimedia/archive/00010/01304pf02_10882a.pdf)
+[[!PMID 11314272]]
diff --git a/bibliography/Chenchik-book-chapter.mdwn b/bibliography/Chenchik-book-chapter.mdwn
new file mode 100644
index 0000000..6091f84
--- /dev/null
+++ b/bibliography/Chenchik-book-chapter.mdwn
@@ -0,0 +1,6 @@
+[[!tag bibliography template_switching]]
+[[!meta date="1 Jan 1998"]]
+
+Chenchik A., Zhu Y.Y., Diatchenko L., Li R., Hill J., Siebert P.D.
+_Gene Cloning and Analysis by RT-PCR._ Siebert P., Larrick J. , editors.
+Natick, MA: BioTechniques Books; **1998**. p. 305-319.

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index 343f078..d2518b4 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-08-15 04:52+0000\n"
+"POT-Creation-Date: 2016-08-15 04:53+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -191,18 +191,19 @@ msgstr ""
 
 #. type: Title ###
 #, no-wrap
-msgid "<a name=\"extra-G\">Does _nanoCAGE_ also add extra _G_s ?</a>"
+msgid "<a name=\"extra-G\">Does _nanoCAGE_ also add extra <em>G</em>s ?</a>"
 msgstr ""
 
 #. type: Plain text
+#, no-wrap
 msgid ""
-"After polymerising the first-strand cDNA, the reverse transcriptase may add "
-"extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra _C_s, "
-"reflected by extra _G_s at the 5′ end of the first-mate sequence reads.  "
-"Some publications suggest that these nucleotides are templated by the RNA "
-"cap itself, which is a methylguanosine.  Thus, by the use of the _template-"
-"switching_ mechanism, nanoCAGE libraries are enriched in 5′-full-length "
-"sequences.  Here are key articles related to this topic:"
+"After polymerising the first-strand cDNA, the reverse transcriptase may add\n"
+"extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra <em>C</em>s,\n"
+"reflected by extra <em>G</em>s at the 5′ end of the first-mate sequence reads.  Some\n"
+"publications suggest that these nucleotides are templated by the RNA cap\n"
+"itself, which is a methylguanosine.  Thus, by the use of the\n"
+"_template-switching_ mechanism, nanoCAGE libraries are enriched in\n"
+"5′-full-length sequences.  Here are key articles related to this topic:\n"
 msgstr ""
 
 #. type: Bullet: ' - '

Use HTML tags.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index f0af7f9..03f912d 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -103,11 +103,11 @@ introducing sample-specific biases ([Tang _et al._,
 2013](http://dx.doi.org/10.1093/nar/gks1128)).
 
 
-### <a name="extra-G">Does _nanoCAGE_ also add extra _G_s ?</a>
+### <a name="extra-G">Does _nanoCAGE_ also add extra <em>G</em>s ?</a>
 
 After polymerising the first-strand cDNA, the reverse transcriptase may add
-extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra _C_s,
-reflected by extra _G_s at the 5′ end of the first-mate sequence reads.  Some
+extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra <em>C</em>s,
+reflected by extra <em>G</em>s at the 5′ end of the first-mate sequence reads.  Some
 publications suggest that these nucleotides are templated by the RNA cap
 itself, which is a methylguanosine.  Thus, by the use of the
 _template-switching_ mechanism, nanoCAGE libraries are enriched in

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index be2cc22..343f078 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-08-15 04:51+0000\n"
+"POT-Creation-Date: 2016-08-15 04:52+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -191,14 +191,14 @@ msgstr ""
 
 #. type: Title ###
 #, no-wrap
-msgid "<a name=\"extra-G\">Does _nanoCAGE_ also add extra `G`s ?</a>"
+msgid "<a name=\"extra-G\">Does _nanoCAGE_ also add extra _G_s ?</a>"
 msgstr ""
 
 #. type: Plain text
 msgid ""
 "After polymerising the first-strand cDNA, the reverse transcriptase may add "
-"extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra `C`s, "
-"reflected by extra `G`s at the 5′ end of the first-mate sequence reads.  "
+"extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra _C_s, "
+"reflected by extra _G_s at the 5′ end of the first-mate sequence reads.  "
 "Some publications suggest that these nucleotides are templated by the RNA "
 "cap itself, which is a methylguanosine.  Thus, by the use of the _template-"
 "switching_ mechanism, nanoCAGE libraries are enriched in 5′-full-length "

Different typography.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 12e6250..f0af7f9 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -103,11 +103,11 @@ introducing sample-specific biases ([Tang _et al._,
 2013](http://dx.doi.org/10.1093/nar/gks1128)).
 
 
-### <a name="extra-G">Does _nanoCAGE_ also add extra `G`s ?</a>
+### <a name="extra-G">Does _nanoCAGE_ also add extra _G_s ?</a>
 
 After polymerising the first-strand cDNA, the reverse transcriptase may add
-extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra `C`s,
-reflected by extra `G`s at the 5′ end of the first-mate sequence reads.  Some
+extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra _C_s,
+reflected by extra _G_s at the 5′ end of the first-mate sequence reads.  Some
 publications suggest that these nucleotides are templated by the RNA cap
 itself, which is a methylguanosine.  Thus, by the use of the
 _template-switching_ mechanism, nanoCAGE libraries are enriched in

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index c9b5e86..be2cc22 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-08-15 04:49+0000\n"
+"POT-Creation-Date: 2016-08-15 04:51+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -207,52 +207,53 @@ msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). Determination of messenger "
-"RNA 5'-ends by reverse transcription of the cap structure]. [Nucleic Acids "
+"Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). _Determination of messenger "
+"RNA 5'-ends by reverse transcription of the cap structure._ [Nucleic Acids "
 "Res. 21, 3597-3598](https://pubmed.gov/8346046), suggesting that the cap is "
 "reverse-transcribed."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). Determination of the "
+"Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). _Determination of the "
 "capped site sequence of mRNA based on the detection of cap-dependent "
-"nucleotide addition using an anchor ligation method. [DNA Res. 11, 305-309]"
+"nucleotide addition using an anchor ligation method._ [DNA Res. 11, 305-309]"
 "(https://pubmed.gov/15500255), showing that A-caps are reverse-transcribed "
 "as Ts."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). Reverse transcriptase "
+"Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). _Reverse transcriptase "
 "template switching: a SMART approach for full-length cDNA library "
-"construction. [BioTechniques 30, 892-897](https://pubmed.gov/11314272), "
+"construction._ [BioTechniques 30, 892-897](https://pubmed.gov/11314272), "
 "showing that nevertheless template switching also occurs on blunt duplexes."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). The human L1 "
+"Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). _The human L1 "
 "promoter: variable transcription initiation sites and a major impact of "
-"upstream flanking sequence on promoter activity. [Genome Res. 2004 "
+"upstream flanking sequence on promoter activity._ [Genome Res. 2004 "
 "Nov;14(11):2253-60](https://pubmed.gov/15520289), showing that endogenous "
 "reverse-transcriptases also reverse-transcribe the cap."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Kulpa D, Topping R, Telesnitsky A. (1997) Determination of the site of first "
-"strand transfer during Moloney murine leukemia virus reverse transcription "
-"and identification of strand transfer-associated reverse transcriptase "
-"errors.  [EMBO J. 1997 Feb 17;16(4):856-65](https://pubmed.gov/9049314), "
-"with another discussion on the reverse-transcription of the cap."
+"Kulpa D, Topping R, Telesnitsky A. (1997) _Determination of the site of "
+"first strand transfer during Moloney murine leukemia virus reverse "
+"transcription and identification of strand transfer-associated reverse "
+"transcriptase errors._ [EMBO J. 1997 Feb 17;16(4):856-65](https://pubmed."
+"gov/9049314), with another discussion on the reverse-transcription of the "
+"cap."
 msgstr ""
 
 #. type: Bullet: ' - '
 msgid ""
-"Oz-Gleenberg I, Herzig E, Hizi A (2011). Template-independent DNA synthesis "
+"Oz-Gleenberg I, Herzig E, Hizi A (2011). _Template-independent DNA synthesis "
 "activity associated with the reverse transcriptase of the long terminal "
-"repeat retrotransposon Tf1. [FEBS J. 2012 Jan;279(1):142-53](https://pubmed."
+"repeat retrotransposon Tf1._ [FEBS J. 2012 Jan;279(1):142-53](https://pubmed."
 "gov/22035236), showith that reverse-transcriptases, like other DNA "
 "polymerases, add non-templated As to blunt DNA duplexes."
 msgstr ""

Titles in italics.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 9249d26..12e6250 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -113,32 +113,32 @@ itself, which is a methylguanosine.  Thus, by the use of the
 _template-switching_ mechanism, nanoCAGE libraries are enriched in
 5′-full-length sequences.  Here are key articles related to this topic:
 
- - Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). Determination of messenger
-   RNA 5'-ends by reverse transcription of the cap structure]. [Nucleic Acids
+ - Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). _Determination of messenger
+   RNA 5'-ends by reverse transcription of the cap structure._ [Nucleic Acids
    Res. 21, 3597-3598](https://pubmed.gov/8346046), suggesting that the cap is
    reverse-transcribed.
- - Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). Determination of the
+ - Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). _Determination of the
    capped site sequence of mRNA based on the detection of cap-dependent
-   nucleotide addition using an anchor ligation method. [DNA Res. 11,
+   nucleotide addition using an anchor ligation method._ [DNA Res. 11,
    305-309](https://pubmed.gov/15500255), showing that A-caps are
    reverse-transcribed as Ts.
- - Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). Reverse transcriptase
+ - Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). _Reverse transcriptase
    template switching: a SMART approach for full-length cDNA library
-   construction. [BioTechniques 30, 892-897](https://pubmed.gov/11314272), showing
+   construction._ [BioTechniques 30, 892-897](https://pubmed.gov/11314272), showing
    that nevertheless template switching also occurs on blunt duplexes.
- - Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). The human L1
+ - Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). _The human L1
    promoter: variable transcription initiation sites and a major impact of
-   upstream flanking sequence on promoter activity. [Genome Res. 2004
+   upstream flanking sequence on promoter activity._ [Genome Res. 2004
    Nov;14(11):2253-60](https://pubmed.gov/15520289), showing that endogenous
    reverse-transcriptases also reverse-transcribe the cap.
- - Kulpa D, Topping R, Telesnitsky A. (1997) Determination of the site of first
+ - Kulpa D, Topping R, Telesnitsky A. (1997) _Determination of the site of first
    strand transfer during Moloney murine leukemia virus reverse transcription
-   and identification of strand transfer-associated reverse transcriptase errors.
+   and identification of strand transfer-associated reverse transcriptase errors._
    [EMBO J. 1997 Feb 17;16(4):856-65](https://pubmed.gov/9049314), with another
    discussion on the reverse-transcription of the cap.
- - Oz-Gleenberg I, Herzig E, Hizi A (2011). Template-independent DNA synthesis
+ - Oz-Gleenberg I, Herzig E, Hizi A (2011). _Template-independent DNA synthesis
    activity associated with the reverse transcriptase of the long terminal
-   repeat retrotransposon Tf1. [FEBS J. 2012
+   repeat retrotransposon Tf1._ [FEBS J. 2012
    Jan;279(1):142-53](https://pubmed.gov/22035236), showith that reverse-transcriptases,
    like other DNA polymerases, add non-templated As to blunt DNA duplexes.
 

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index f371247..c9b5e86 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-03-29 06:19+0000\n"
+"POT-Creation-Date: 2016-08-15 04:49+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -191,6 +191,74 @@ msgstr ""
 
 #. type: Title ###
 #, no-wrap
+msgid "<a name=\"extra-G\">Does _nanoCAGE_ also add extra `G`s ?</a>"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"After polymerising the first-strand cDNA, the reverse transcriptase may add "
+"extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra `C`s, "
+"reflected by extra `G`s at the 5′ end of the first-mate sequence reads.  "
+"Some publications suggest that these nucleotides are templated by the RNA "
+"cap itself, which is a methylguanosine.  Thus, by the use of the _template-"
+"switching_ mechanism, nanoCAGE libraries are enriched in 5′-full-length "
+"sequences.  Here are key articles related to this topic:"
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). Determination of messenger "
+"RNA 5'-ends by reverse transcription of the cap structure]. [Nucleic Acids "
+"Res. 21, 3597-3598](https://pubmed.gov/8346046), suggesting that the cap is "
+"reverse-transcribed."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). Determination of the "
+"capped site sequence of mRNA based on the detection of cap-dependent "
+"nucleotide addition using an anchor ligation method. [DNA Res. 11, 305-309]"
+"(https://pubmed.gov/15500255), showing that A-caps are reverse-transcribed "
+"as Ts."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). Reverse transcriptase "
+"template switching: a SMART approach for full-length cDNA library "
+"construction. [BioTechniques 30, 892-897](https://pubmed.gov/11314272), "
+"showing that nevertheless template switching also occurs on blunt duplexes."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). The human L1 "
+"promoter: variable transcription initiation sites and a major impact of "
+"upstream flanking sequence on promoter activity. [Genome Res. 2004 "
+"Nov;14(11):2253-60](https://pubmed.gov/15520289), showing that endogenous "
+"reverse-transcriptases also reverse-transcribe the cap."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Kulpa D, Topping R, Telesnitsky A. (1997) Determination of the site of first "
+"strand transfer during Moloney murine leukemia virus reverse transcription "
+"and identification of strand transfer-associated reverse transcriptase "
+"errors.  [EMBO J. 1997 Feb 17;16(4):856-65](https://pubmed.gov/9049314), "
+"with another discussion on the reverse-transcription of the cap."
+msgstr ""
+
+#. type: Bullet: ' - '
+msgid ""
+"Oz-Gleenberg I, Herzig E, Hizi A (2011). Template-independent DNA synthesis "
+"activity associated with the reverse transcriptase of the long terminal "
+"repeat retrotransposon Tf1. [FEBS J. 2012 Jan;279(1):142-53](https://pubmed."
+"gov/22035236), showith that reverse-transcriptases, like other DNA "
+"polymerases, add non-templated As to blunt DNA duplexes."
+msgstr ""
+
+#. type: Title ###
+#, no-wrap
 msgid "<a name=\"CAGEscan\">What is the difference between _nanoCAGE_ and _CAGEscan_ ?</a>"
 msgstr ""
 

Add bibliography related to G-addition.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 68d6828..9249d26 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -103,6 +103,45 @@ introducing sample-specific biases ([Tang _et al._,
 2013](http://dx.doi.org/10.1093/nar/gks1128)).
 
 
+### <a name="extra-G">Does _nanoCAGE_ also add extra `G`s ?</a>
+
+After polymerising the first-strand cDNA, the reverse transcriptase may add
+extra nucleotides.  In _nanoCAGE_ we observe a prevalence of extra `C`s,
+reflected by extra `G`s at the 5′ end of the first-mate sequence reads.  Some
+publications suggest that these nucleotides are templated by the RNA cap
+itself, which is a methylguanosine.  Thus, by the use of the
+_template-switching_ mechanism, nanoCAGE libraries are enriched in
+5′-full-length sequences.  Here are key articles related to this topic:
+
+ - Hirzmann, J., Luo, D., Hahnen, J. et al. (1993). Determination of messenger
+   RNA 5'-ends by reverse transcription of the cap structure]. [Nucleic Acids
+   Res. 21, 3597-3598](https://pubmed.gov/8346046), suggesting that the cap is
+   reverse-transcribed.
+ - Ohake, H., Ohtoko, K., Ishimaru, Y. et al. (2004). Determination of the
+   capped site sequence of mRNA based on the detection of cap-dependent
+   nucleotide addition using an anchor ligation method. [DNA Res. 11,
+   305-309](https://pubmed.gov/15500255), showing that A-caps are
+   reverse-transcribed as Ts.
+ - Zhu, YY., Machleder, EM., Chenchik, A. et al. (2001). Reverse transcriptase
+   template switching: a SMART approach for full-length cDNA library
+   construction. [BioTechniques 30, 892-897](https://pubmed.gov/11314272), showing
+   that nevertheless template switching also occurs on blunt duplexes.
+ - Lavie L, Maldener E, Brouha B, Meese EU, Mayer J. (2004). The human L1
+   promoter: variable transcription initiation sites and a major impact of
+   upstream flanking sequence on promoter activity. [Genome Res. 2004
+   Nov;14(11):2253-60](https://pubmed.gov/15520289), showing that endogenous
+   reverse-transcriptases also reverse-transcribe the cap.
+ - Kulpa D, Topping R, Telesnitsky A. (1997) Determination of the site of first
+   strand transfer during Moloney murine leukemia virus reverse transcription
+   and identification of strand transfer-associated reverse transcriptase errors.
+   [EMBO J. 1997 Feb 17;16(4):856-65](https://pubmed.gov/9049314), with another
+   discussion on the reverse-transcription of the cap.
+ - Oz-Gleenberg I, Herzig E, Hizi A (2011). Template-independent DNA synthesis
+   activity associated with the reverse transcriptase of the long terminal
+   repeat retrotransposon Tf1. [FEBS J. 2012
+   Jan;279(1):142-53](https://pubmed.gov/22035236), showith that reverse-transcriptases,
+   like other DNA polymerases, add non-templated As to blunt DNA duplexes.
+
 ### <a name="CAGEscan">What is the difference between _nanoCAGE_ and _CAGEscan_ ?</a>
 
 _nanoCAGE_ is a CAGE protocol using _template switching_ to enrich for 5′ ends.

updated PO files
diff --git a/News/2015/IMGC_2015.ja.po b/News/2015/IMGC_2015.ja.po
index cfc228f..4497af8 100644
--- a/News/2015/IMGC_2015.ja.po
+++ b/News/2015/IMGC_2015.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-11-17 05:33+0000\n"
+"POT-Creation-Date: 2016-04-26 01:41+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -35,8 +35,7 @@ msgstr ""
 #, no-wrap
 msgid ""
 "On November 9<sup>th</sup> [[People/Charles_Plessy]] gave a talk\n"
-"on [_Single-molecule RNA sequencing in single "
-"cells_](http://imgc2015.jp/abstracts.html#O-12)\n"
+"on [_Single-molecule RNA sequencing in single cells_](http://imgs.org/Archive/abstracts/2015Abstracts/IMGC2015program.html#O-12)\n"
 "at the International Mammalian Genomics Conference \n"
 "([IMGC](http://imgc2015.jp/))in Yokohama, Japan.\n"
 msgstr ""

Use archive link.
diff --git a/News/2015/IMGC_2015.mdwn b/News/2015/IMGC_2015.mdwn
index 6a6d1e1..4e2c8eb 100644
--- a/News/2015/IMGC_2015.mdwn
+++ b/News/2015/IMGC_2015.mdwn
@@ -3,6 +3,6 @@
 [[!tag Meeting People/Charles_Plessy]]
 
 On November 9<sup>th</sup> [[People/Charles_Plessy]] gave a talk
-on [_Single-molecule RNA sequencing in single cells_](http://imgc2015.jp/abstracts.html#O-12)
+on [_Single-molecule RNA sequencing in single cells_](http://imgs.org/Archive/abstracts/2015Abstracts/IMGC2015program.html#O-12)
 at the International Mammalian Genomics Conference 
 ([IMGC](http://imgc2015.jp/))in Yokohama, Japan.

Corresponding author.
diff --git a/bibliography/27071605.mdwn b/bibliography/27071605.mdwn
index bcf5577..30c5ef8 100644
--- a/bibliography/27071605.mdwn
+++ b/bibliography/27071605.mdwn
@@ -1,7 +1,7 @@
 [[!tag bibliography People/Charles_Plessy]]
 [[!meta date="01 Apr 2016"]]
 
-Arnaud O, Kato S, Poulain S, Plessy C.
+Arnaud O, Kato S, Poulain S, Plessy C<sup>✉</sup>.
 Targeted reduction of highly abundant transcripts using pseudo-random primers.
 [[!doi 10.2144/000114400 desc="Biotechniques. **2016** Apr 1;60(4):169-74."]]
 [[!doi 10.1101/027805 desc="bioRxiv"]]

Correct DOI.
diff --git a/bibliography/27071605.mdwn b/bibliography/27071605.mdwn
index 4879bba..bcf5577 100644
--- a/bibliography/27071605.mdwn
+++ b/bibliography/27071605.mdwn
@@ -4,5 +4,5 @@
 Arnaud O, Kato S, Poulain S, Plessy C.
 Targeted reduction of highly abundant transcripts using pseudo-random primers.
 [[!doi 10.2144/000114400 desc="Biotechniques. **2016** Apr 1;60(4):169-74."]]
-[[!doi http://dx.doi.org/10.1101/027805 desc="bioRxiv"]]
+[[!doi 10.1101/027805 desc="bioRxiv"]]
 [[!PMID 27071605]]

updated PO files
diff --git a/Publications/PseudoRandom-2016.ja.po b/Publications/PseudoRandom-2016.ja.po
index 377875d..74284e1 100644
--- a/Publications/PseudoRandom-2016.ja.po
+++ b/Publications/PseudoRandom-2016.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-04-14 06:52+0000\n"
+"POT-Creation-Date: 2016-04-15 05:19+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -90,11 +90,6 @@ msgid "Reference:"
 msgstr ""
 
 #. type: Plain text
-msgid ""
-"Ophélie Arnaud, Sachi Kato, Stéphane Poulain, and Charles Plessy.  Targeted "
-"reduction of highly abundant transcripts using pseudo-random primers.  "
-"[BioTechniques, Vol. 60, No. 4, April 2016, pp. 169–174](http://www."
-"biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-"
-"highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067."
-"html).  doi:10.2144/000114400 Pubmed registration in progress."
+#, no-wrap
+msgid "[[!inline pages=bibliography/27071605 template=biblio feeds=no show=0]]\n"
 msgstr ""

Pseudo-random primers Now on PubMed.
diff --git a/Publications/PseudoRandom-2016.mdwn b/Publications/PseudoRandom-2016.mdwn
index 61d9350..78a6402 100644
--- a/Publications/PseudoRandom-2016.mdwn
+++ b/Publications/PseudoRandom-2016.mdwn
@@ -40,7 +40,4 @@ material in GitHub](https://github.com/Population-Transcriptomics/pseudo-random-
 ----
 Reference:
 
-Ophélie Arnaud, Sachi Kato, Stéphane Poulain, and Charles Plessy.  Targeted
-reduction of highly abundant transcripts using pseudo-random primers.
-[BioTechniques, Vol. 60, No. 4, April 2016, pp. 169–174](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html).
-doi:10.2144/000114400 Pubmed registration in progress.
+[[!inline pages=bibliography/27071605 template=biblio feeds=no show=0]]
diff --git a/bibliography/27071605.mdwn b/bibliography/27071605.mdwn
new file mode 100644
index 0000000..4879bba
--- /dev/null
+++ b/bibliography/27071605.mdwn
@@ -0,0 +1,8 @@
+[[!tag bibliography People/Charles_Plessy]]
+[[!meta date="01 Apr 2016"]]
+
+Arnaud O, Kato S, Poulain S, Plessy C.
+Targeted reduction of highly abundant transcripts using pseudo-random primers.
+[[!doi 10.2144/000114400 desc="Biotechniques. **2016** Apr 1;60(4):169-74."]]
+[[!doi http://dx.doi.org/10.1101/027805 desc="bioRxiv"]]
+[[!PMID 27071605]]

updated PO files
diff --git a/News/2016.ja.po b/News/2016.ja.po
new file mode 100644
index 0000000..a380738
--- /dev/null
+++ b/News/2016.ja.po
@@ -0,0 +1,22 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2016-04-14 06:57+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta redir=News]]\n"
+msgstr ""
diff --git a/News/2016/new_member_february.ja.po b/News/2016/new_member_february.ja.po
new file mode 100644
index 0000000..78aa945
--- /dev/null
+++ b/News/2016/new_member_february.ja.po
@@ -0,0 +1,38 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2016-04-14 06:57+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Welcome to Simon Besson-Girard\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"1 Feb 2016\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag People/Simon_Besson_Girard]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"We are happy to welcome Simon Besson-Girard, a student of the University of "
+"Lyon, France, who visits us to analyse our single-cell data."
+msgstr ""

Welcome Simon.
diff --git a/News/2016.mdwn b/News/2016.mdwn
new file mode 100644
index 0000000..1174d17
--- /dev/null
+++ b/News/2016.mdwn
@@ -0,0 +1 @@
+[[!meta redir=News]]
diff --git a/News/2016/new_member_february.mdwn b/News/2016/new_member_february.mdwn
new file mode 100644
index 0000000..5fac7df
--- /dev/null
+++ b/News/2016/new_member_february.mdwn
@@ -0,0 +1,6 @@
+[[!meta title="Welcome to Simon Besson-Girard"]]
+[[!meta date="1 Feb 2016"]]
+[[!tag People/Simon_Besson_Girard]]
+
+We are happy to welcome Simon Besson-Girard, a student of the University of
+Lyon, France, who visits us to analyse our single-cell data.

updated PO files
diff --git a/Publications/PseudoRandom-2016.ja.po b/Publications/PseudoRandom-2016.ja.po
index 029e658..377875d 100644
--- a/Publications/PseudoRandom-2016.ja.po
+++ b/Publications/PseudoRandom-2016.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-04-14 06:46+0000\n"
+"POT-Creation-Date: 2016-04-14 06:52+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -55,9 +55,9 @@ msgid ""
 "different primers, that covers all the possible combinations of A, C, G and "
 "T on 6 consecutive nucleotides.  In 2009, [Armour and collaborators](http://"
 "dx.doi.org/10.1038/nmeth.1360) showed that by using a subset of these "
-"\"random\" primers, on can avoid the conversion of target RNA molecules "
-"(typically the ribosomal RNAs, which carry little information compared to "
-"their abundance).  However, this subset was several hundreds of "
+"\"random\" primers, one can avoid the conversion of target RNA molecules "
+"(typically the ribosomal RNAs, which are highly abundant but carry little "
+"information).  However, this subset was several hundreds of "
 "oligonucleotides, which is costly to synthesize."
 msgstr ""
 
@@ -66,10 +66,10 @@ msgid ""
 "Observing that the reverse-transcriptase is highly tolerant to mismatches, "
 "we reasoned that a similar result could be obtained with a dramatically "
 "lower number of oligonucleotides, which we called \"_pseudo-random_ primers"
-"\".  In an article published this month in BioTechniques [Arnaud et al., "
+"\".  In an article published this month in BioTechniques ([Arnaud et al., "
 "2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-"
 "reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/"
-"biotechniques-364067.html), we demonstrate our approach by reducing either "
+"biotechniques-364067.html)), we demonstrate our approach by reducing either "
 "ribosomal or hemoglobin RNA.  Importantly, our method also applies to the "
 "reduction of the artifacts created by the cross priming of the "
 "oligonucleotide tails."
@@ -77,12 +77,12 @@ msgstr ""
 
 #. type: Plain text
 msgid ""
-"During the publication process of this work, we also explored the modern "
-"ways of communicating results.  We deposited our presubmission manuscript to "
-"the \"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)\" "
-"repository, and the scripts of our bioinformatics analysis as [supplemental "
-"material in GitHub](https://github.com/Population-Transcriptomics/pseudo-"
-"random-primers)."
+"During the publication process of this work, we also explored the new ways "
+"of communicating scientific results on Internet.  We deposited our "
+"[[presubmission manuscript|News/2015/pseudo-random]] to the \"[bioRxiv]"
+"(http://www.biorxiv.org/content/early/2015/09/29/027805)\" repository, and "
+"the scripts of our bioinformatics analysis as [supplemental material in "
+"GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers)."
 msgstr ""
 
 #. type: Plain text

Tweaks.
diff --git a/Publications/PseudoRandom-2016.mdwn b/Publications/PseudoRandom-2016.mdwn
index 7a279c4..61d9350 100644
--- a/Publications/PseudoRandom-2016.mdwn
+++ b/Publications/PseudoRandom-2016.mdwn
@@ -18,24 +18,24 @@ synthesis primers.  Reactions to convert the whole transcriptome are often
 conduced with a "random" mixture of 4,096 different primers, that covers all
 the possible combinations of A, C, G and T on 6 consecutive nucleotides.  In
 2009, [Armour and collaborators](http://dx.doi.org/10.1038/nmeth.1360) showed
-that by using a subset of these "random" primers, on can avoid the conversion
-of target RNA molecules (typically the ribosomal RNAs, which carry little
-information compared to their abundance).  However, this subset was several
+that by using a subset of these "random" primers, one can avoid the conversion
+of target RNA molecules (typically the ribosomal RNAs, which are highly
+abundant but carry little information).  However, this subset was several
 hundreds of oligonucleotides, which is costly to synthesize.
 
 Observing that the reverse-transcriptase is highly tolerant to mismatches,
 we reasoned that a similar result could be obtained with a dramatically
 lower number of oligonucleotides, which we called "_pseudo-random_ primers".
-In an article published this month in BioTechniques [Arnaud et al., 2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html),
+In an article published this month in BioTechniques ([Arnaud et al., 2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html)),
 we demonstrate our approach by reducing either ribosomal or hemoglobin RNA.
 Importantly, our method also applies to the reduction of the artifacts
 created by the cross priming of the oligonucleotide tails.
 
-During the publication process of this work, we also explored the modern ways
-of communicating results.  We deposited our presubmission manuscript to the
-"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)" repository,
-and the scripts of our bioinformatics analysis as
-[supplemental material in GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers).
+During the publication process of this work, we also explored the new ways of
+communicating scientific results on Internet.  We deposited our [[presubmission
+manuscript|News/2015/pseudo-random]] to the "[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)"
+repository, and the scripts of our bioinformatics analysis as [supplemental
+material in GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers).
 
 ----
 Reference:

updated PO files
diff --git a/Publications/PseudoRandom-2016.ja.po b/Publications/PseudoRandom-2016.ja.po
index 7539fc8..029e658 100644
--- a/Publications/PseudoRandom-2016.ja.po
+++ b/Publications/PseudoRandom-2016.ja.po
@@ -18,21 +18,17 @@ msgstr ""
 
 #. type: Plain text
 #, no-wrap
-msgid ""
-"[[!meta title=\"Targeted reduction of highly abundant transcripts with "
-"pseudo-random primers.\"]]\n"
+msgid "[[!meta title=\"Targeted reduction of highly abundant transcripts with pseudo-random primers.\"]]\n"
 msgstr ""
 
 #. type: Plain text
 #, no-wrap
-msgid "[[!meta date=\"6 Jun 2014\"]]\n"
+msgid "[[!meta date=\"14 Apr 2016\"]]\n"
 msgstr ""
 
 #. type: Title =
 #, no-wrap
-msgid ""
-"Targeted reduction of highly abundant transcripts with pseudo-random "
-"primers\n"
+msgid "Targeted reduction of highly abundant transcripts with pseudo-random primers\n"
 msgstr ""
 
 #. type: Plain text
@@ -57,11 +53,11 @@ msgid ""
 "oligonucleotides as synthesis primers.  Reactions to convert the whole "
 "transcriptome are often conduced with a \"random\" mixture of 4,096 "
 "different primers, that covers all the possible combinations of A, C, G and "
-"T on 6 consecutive nucleotides.  In 2009, [Armour and "
-"collaborators](http://dx.doi.org/10.1038/nmeth.1360) showed that by using a "
-"subset of these \"random\" primers, on can avoid the conversion of target "
-"RNA molecules (typically the ribosomal RNAs, which carry little information "
-"compared to their abundance).  However, this subset was several hundreds of "
+"T on 6 consecutive nucleotides.  In 2009, [Armour and collaborators](http://"
+"dx.doi.org/10.1038/nmeth.1360) showed that by using a subset of these "
+"\"random\" primers, on can avoid the conversion of target RNA molecules "
+"(typically the ribosomal RNAs, which carry little information compared to "
+"their abundance).  However, this subset was several hundreds of "
 "oligonucleotides, which is costly to synthesize."
 msgstr ""
 
@@ -69,13 +65,14 @@ msgstr ""
 msgid ""
 "Observing that the reverse-transcriptase is highly tolerant to mismatches, "
 "we reasoned that a similar result could be obtained with a dramatically "
-"lower number of oligonucleotides, which we called \"_pseudo-random_ "
-"primers\".  In an article published this month in BioTechniques [Arnaud et "
-"al., "
-"2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html), "
-"we demonstrate our approach by reducing either ribosomal or hemoglobin RNA.  "
-"Importantly, our method also applies to the reduction of the artifacts "
-"created by the cross priming of the oligonucleotide tails."
+"lower number of oligonucleotides, which we called \"_pseudo-random_ primers"
+"\".  In an article published this month in BioTechniques [Arnaud et al., "
+"2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-"
+"reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/"
+"biotechniques-364067.html), we demonstrate our approach by reducing either "
+"ribosomal or hemoglobin RNA.  Importantly, our method also applies to the "
+"reduction of the artifacts created by the cross priming of the "
+"oligonucleotide tails."
 msgstr ""
 
 #. type: Plain text
@@ -84,8 +81,8 @@ msgid ""
 "ways of communicating results.  We deposited our presubmission manuscript to "
 "the \"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)\" "
 "repository, and the scripts of our bioinformatics analysis as [supplemental "
-"material in "
-"GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers)."
+"material in GitHub](https://github.com/Population-Transcriptomics/pseudo-"
+"random-primers)."
 msgstr ""
 
 #. type: Plain text
@@ -96,8 +93,8 @@ msgstr ""
 msgid ""
 "Ophélie Arnaud, Sachi Kato, Stéphane Poulain, and Charles Plessy.  Targeted "
 "reduction of highly abundant transcripts using pseudo-random primers.  "
-"[BioTechniques, Vol. 60, No. 4, April 2016, "
-"pp. "
-"169–174](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html).  "
-"doi:10.2144/000114400 Pubmed registration in progress."
+"[BioTechniques, Vol. 60, No. 4, April 2016, pp. 169–174](http://www."
+"biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-"
+"highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067."
+"html).  doi:10.2144/000114400 Pubmed registration in progress."
 msgstr ""

Corrected date.
diff --git a/Publications/PseudoRandom-2016.mdwn b/Publications/PseudoRandom-2016.mdwn
index 7d77c34..7a279c4 100644
--- a/Publications/PseudoRandom-2016.mdwn
+++ b/Publications/PseudoRandom-2016.mdwn
@@ -1,5 +1,5 @@
 [[!meta title="Targeted reduction of highly abundant transcripts with pseudo-random primers."]]
-[[!meta date="6 Jun 2014"]]
+[[!meta date="14 Apr 2016"]]
 
 Targeted reduction of highly abundant transcripts with pseudo-random primers
 ============================================================================

updated PO files
diff --git a/Publications/PseudoRandom-2016.ja.po b/Publications/PseudoRandom-2016.ja.po
new file mode 100644
index 0000000..7539fc8
--- /dev/null
+++ b/Publications/PseudoRandom-2016.ja.po
@@ -0,0 +1,103 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2016-04-14 06:46+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!meta title=\"Targeted reduction of highly abundant transcripts with "
+"pseudo-random primers.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"6 Jun 2014\"]]\n"
+msgstr ""
+
+#. type: Title =
+#, no-wrap
+msgid ""
+"Targeted reduction of highly abundant transcripts with pseudo-random "
+"primers\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"In quantitative analysis of gene expression using DNA sequencers, the "
+"precision depends on the number of sequence reads, and each of these reads "
+"has a cost.  Therefore, if one prevents uninformative sequences to be read, "
+"the cost/performance of the analysis increases.  We have developed a method "
+"to deplete target sequences from transcriptome libraries (typically "
+"[[nanoCAGE]])."
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "<img src=\"/images/PseudoRandomPrimers.png\" width=\"100%\">\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Before sequencing, RNA molecules are usually converted into DNA molecules "
+"with an enzyme, the reverse-transcriptase, that uses short DNA "
+"oligonucleotides as synthesis primers.  Reactions to convert the whole "
+"transcriptome are often conduced with a \"random\" mixture of 4,096 "
+"different primers, that covers all the possible combinations of A, C, G and "
+"T on 6 consecutive nucleotides.  In 2009, [Armour and "
+"collaborators](http://dx.doi.org/10.1038/nmeth.1360) showed that by using a "
+"subset of these \"random\" primers, on can avoid the conversion of target "
+"RNA molecules (typically the ribosomal RNAs, which carry little information "
+"compared to their abundance).  However, this subset was several hundreds of "
+"oligonucleotides, which is costly to synthesize."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Observing that the reverse-transcriptase is highly tolerant to mismatches, "
+"we reasoned that a similar result could be obtained with a dramatically "
+"lower number of oligonucleotides, which we called \"_pseudo-random_ "
+"primers\".  In an article published this month in BioTechniques [Arnaud et "
+"al., "
+"2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html), "
+"we demonstrate our approach by reducing either ribosomal or hemoglobin RNA.  "
+"Importantly, our method also applies to the reduction of the artifacts "
+"created by the cross priming of the oligonucleotide tails."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"During the publication process of this work, we also explored the modern "
+"ways of communicating results.  We deposited our presubmission manuscript to "
+"the \"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)\" "
+"repository, and the scripts of our bioinformatics analysis as [supplemental "
+"material in "
+"GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers)."
+msgstr ""
+
+#. type: Plain text
+msgid "Reference:"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Ophélie Arnaud, Sachi Kato, Stéphane Poulain, and Charles Plessy.  Targeted "
+"reduction of highly abundant transcripts using pseudo-random primers.  "
+"[BioTechniques, Vol. 60, No. 4, April 2016, "
+"pp. "
+"169–174](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html).  "
+"doi:10.2144/000114400 Pubmed registration in progress."
+msgstr ""

Draft news entry.
diff --git a/Publications/PseudoRandom-2016.mdwn b/Publications/PseudoRandom-2016.mdwn
new file mode 100644
index 0000000..7d77c34
--- /dev/null
+++ b/Publications/PseudoRandom-2016.mdwn
@@ -0,0 +1,46 @@
+[[!meta title="Targeted reduction of highly abundant transcripts with pseudo-random primers."]]
+[[!meta date="6 Jun 2014"]]
+
+Targeted reduction of highly abundant transcripts with pseudo-random primers
+============================================================================
+
+In quantitative analysis of gene expression using DNA sequencers, the precision
+depends on the number of sequence reads, and each of these reads has a cost.
+Therefore, if one prevents uninformative sequences to be read, the
+cost/performance of the analysis increases.  We have developed a method to
+deplete target sequences from transcriptome libraries (typically [[nanoCAGE]]).
+
+<img src="/images/PseudoRandomPrimers.png" width="100%">
+
+Before sequencing, RNA molecules are usually converted into DNA molecules with
+an enzyme, the reverse-transcriptase, that uses short DNA oligonucleotides as
+synthesis primers.  Reactions to convert the whole transcriptome are often
+conduced with a "random" mixture of 4,096 different primers, that covers all
+the possible combinations of A, C, G and T on 6 consecutive nucleotides.  In
+2009, [Armour and collaborators](http://dx.doi.org/10.1038/nmeth.1360) showed
+that by using a subset of these "random" primers, on can avoid the conversion
+of target RNA molecules (typically the ribosomal RNAs, which carry little
+information compared to their abundance).  However, this subset was several
+hundreds of oligonucleotides, which is costly to synthesize.
+
+Observing that the reverse-transcriptase is highly tolerant to mismatches,
+we reasoned that a similar result could be obtained with a dramatically
+lower number of oligonucleotides, which we called "_pseudo-random_ primers".
+In an article published this month in BioTechniques [Arnaud et al., 2016](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html),
+we demonstrate our approach by reducing either ribosomal or hemoglobin RNA.
+Importantly, our method also applies to the reduction of the artifacts
+created by the cross priming of the oligonucleotide tails.
+
+During the publication process of this work, we also explored the modern ways
+of communicating results.  We deposited our presubmission manuscript to the
+"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)" repository,
+and the scripts of our bioinformatics analysis as
+[supplemental material in GitHub](https://github.com/Population-Transcriptomics/pseudo-random-primers).
+
+----
+Reference:
+
+Ophélie Arnaud, Sachi Kato, Stéphane Poulain, and Charles Plessy.  Targeted
+reduction of highly abundant transcripts using pseudo-random primers.
+[BioTechniques, Vol. 60, No. 4, April 2016, pp. 169–174](http://www.biotechniques.com/BiotechniquesJournal/2016/April/Targeted-reduction-of-highly-abundant-transcripts-using-pseudo-random-primers/biotechniques-364067.html).
+doi:10.2144/000114400 Pubmed registration in progress.

Correct typo and slightly reduce font size.
diff --git a/images/PseudoRandomPrimers.png b/images/PseudoRandomPrimers.png
index 56287e3..7482cfe 100644
Binary files a/images/PseudoRandomPrimers.png and b/images/PseudoRandomPrimers.png differ
diff --git a/images/PseudoRandomPrimers.svg b/images/PseudoRandomPrimers.svg
index bb61edd..8b1552b 100644
--- a/images/PseudoRandomPrimers.svg
+++ b/images/PseudoRandomPrimers.svg
@@ -33,8 +33,8 @@
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+     inkscape:cy="32.582061"
      inkscape:window-x="0"
      inkscape:window-y="30"
      inkscape:window-maximized="1"
@@ -2082,265 +2082,267 @@
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+       y="-554.61334"
+       x="2102.7444"
+       style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:69.27076721px;line-height:125%;font-family:Serif;-inkscape-font-specification:Serif;text-align:center;letter-spacing:0px;word-spacing:0px;text-anchor:middle;fill:#000000;fill-opacity:1;stroke:none"
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-         style="font-style:italic;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:45.59764862px;font-family:Utopia;-inkscape-font-specification:'Utopia Italic';text-align:center;text-anchor:middle;fill:#4d4d4d;fill-opacity:1"
-         y="-568.28314"
-         x="2090.8135"
+         style="font-style:italic;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:44.53120422px;font-family:Utopia;-inkscape-font-specification:'Utopia Italic';text-align:center;text-anchor:middle;fill:#4d4d4d;fill-opacity:1"
+         y="-554.61334"
+         x="2102.7444"
          sodipodi:role="line">Pseudo-random primers</tspan></text>
 <ellipse
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+       style="fill:#ffffff;fill-opacity:1;stroke:#000000;stroke-width:1.89479458;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
        id="path4315"
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-       cy="-418.97318"
-       rx="100.39772"
-       ry="102.25694" /><ellipse
-       style="fill:#ff2a2a;fill-opacity:1;stroke:#ffffff;stroke-width:1.9401716;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
+       cx="2292.5903"
+       cy="-419.13672"
+       rx="98.049606"
+       ry="99.865341" /><ellipse
+       style="fill:#ff2a2a;fill-opacity:1;stroke:#ffffff;stroke-width:1.89479458;stroke-linejoin:round;stroke-miterlimit:4;stroke-dasharray:none;stroke-opacity:1"
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        transform="scale(1,-1)"
-       cx="2302.2046"
-       cy="-418.97324"
-       rx="76.227898"
-       ry="63.213379" /><circle
+       cx="2305.3003"
+       cy="-419.13678"
+       rx="74.445068"
+       ry="61.734936" /><circle
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        id="path4319"
        transform="scale(1,-1)"
-       cx="2330.0928"
-       cy="-421.76196"
-       r="15.803345" /><circle
+       cx="2332.5364"
+       cy="-421.86029"
+       r="15.433734" /><circle
        transform="scale(1,-1)"
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        style="fill:#2a7fff;fill-opacity:1;stroke:none"
-       cx="2296.627"
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-       r="15.803345" /><circle
+       cx="2299.8533"
+       cy="-390.99292"
+       r="15.433734" /><circle
        style="fill:#ab37c8;fill-opacity:1;stroke:none"
        id="path4323"
        transform="scale(1,-1)"
-       cx="2278.9646"
-       cy="-339.95645"
-       r="15.803345" /><text
+       cx="2282.604"
+       cy="-341.96805"
+       r="15.433734" /><text
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        sodipodi:linespacing="125%"
        id="text4325"
-       y="-411.91217"
-       x="1847.4521"
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+       y="-405.27194"
+       x="1861.2141"
+       style="font-style:normal;font-weight:normal;font-size:25.87151146px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#000000;fill-opacity:1;stroke:none"
        xml:space="preserve"><tspan
          style="fill:#000000"
-         y="-411.91217"
-         x="1847.4521"
+         y="-405.27194"
+         x="1861.2141"
          id="tspan4327"
          sodipodi:role="line">Usually, the whole</tspan><tspan
          id="tspan4627"
          style="fill:#000000"
-         y="-373.44144"
-         x="1847.4521"
+         y="-372.93256"
+         x="1861.2141"
          sodipodi:role="line">transcriptome is</tspan><tspan
          id="tspan4630"
          style="fill:#000000"
-         y="-334.97073"
-         x="1847.4521"
+         y="-340.59317"
+         x="1861.2141"
          sodipodi:role="line">reverse-transcribed</tspan><tspan
          style="fill:#000000"
-         y="-296.5"
-         x="1847.4521"
+         y="-308.25378"
+         x="1861.2141"
          sodipodi:role="line"
          id="tspan4333">with 4096 <tspan
    style="font-style:italic;-inkscape-font-specification:'Sans Italic';fill:#000000"
    id="tspan4329">random</tspan></tspan><tspan
          id="tspan4642"
          style="fill:#000000"
-         y="-258.02927"
-         x="1847.4521"
+         y="-275.9144"
+         x="1861.2141"
          sodipodi:role="line">(NNNNNN) primers.</tspan></text>
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        xml:space="preserve"
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-       x="-1848.3787"
-       y="-374.44812"
+       style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:111.48309326px;line-height:125%;font-family:Serif;-inkscape-font-specification:Serif;letter-spacing:0px;word-spacing:0px;fill:#ffffff;fill-opacity:1;stroke:#000000;stroke-width:0.87739444"
+       x="-1853.1144"
+       y="-375.47284"
        id="text4335"
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        transform="matrix(-1.13404,-0.37021934,-0.26014824,0.79687498,0,0)"
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-       inkscape:transform-center-y="-46.059114"><tspan
+       inkscape:transform-center-x="-19.16309"
+       inkscape:transform-center-y="-44.981887"><tspan
          sodipodi:role="line"
-         x="-1848.3787"
-         y="-374.44812"
-         style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:73.3840332px;font-family:Sans;-inkscape-font-specification:Sans;fill:#ffffff;stroke:#000000;stroke-width:0.89840645"
+         x="-1853.1144"
+         y="-375.47284"
+         style="font-style:normal;font-variant:normal;font-weight:normal;font-stretch:normal;font-size:71.66771698px;font-family:Sans;-inkscape-font-specification:Sans;fill:#ffffff;stroke:#000000;stroke-width:0.87739444"
          id="tspan4337">→</tspan></text>
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        xml:space="preserve"
-       style="font-style:normal;font-weight:normal;font-size:27.64987755px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#ff2a2a;fill-opacity:1;stroke:none"
-       x="2405.825"
-       y="-589.28052"
+       style="font-style:normal;font-weight:normal;font-size:25.87151146px;line-height:125%;font-family:Sans;letter-spacing:0px;word-spacing:0px;fill:#ff2a2a;fill-opacity:1;stroke:none"
+       x="2423.0518"
+       y="-590.63153"
        id="text4339"
        sodipodi:linespacing="125%"><tspan
          id="tspan4341"
          sodipodi:role="line"
-         x="2405.825"
-         y="-589.28052"
+         x="2423.0518"
+         y="-590.63153"
          style="fill:#ff2a2a;fill-opacity:1">A subset of ~700 <tspan
    style="font-style:italic;-inkscape-font-specification:'Sans Italic'"
    id="tspan4343">not-so</tspan></tspan><tspan
          sodipodi:role="line"
-         x="2405.825"
-         y="-550.80981"
+         x="2423.0518"
+         y="-558.29211"
          style="fill:#ff2a2a;fill-opacity:1"
          id="tspan4345"><tspan
    style="font-style:italic;-inkscape-font-specification:'Sans Italic'"
    id="tspan4347">random</tspan> primers cover the</tspan><tspan
          sodipodi:role="line"
-         x="2405.825"
-         y="-512.33905"
+         x="2423.0518"
+         y="-525.95276"
          style="fill:#ff2a2a;fill-opacity:1"
          id="tspan4349">whole transcriptome, while</tspan><tspan
          sodipodi:role="line"
-         x="2405.825"
-         y="-473.86835"
+         x="2423.0518"
+         y="-493.6134"

(Diff truncated)
updated PO files
diff --git a/index.ja.po b/index.ja.po
index 0bff4cf..26cb867 100644
--- a/index.ja.po
+++ b/index.ja.po
@@ -2,7 +2,7 @@
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 "Project-Id-Version: \n"
-"POT-Creation-Date: 2015-01-17 12:25+0000\n"
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 "PO-Revision-Date: 2014-06-06 09:59+0900\n"
 "Last-Translator: Charles Plessy <plessy@riken.jp>\n"
 "Language-Team: GMTU\n"
@@ -74,14 +74,32 @@ msgid "</nav>\n"
 msgstr "</nav>\n"
 
 #. type: Plain text
-#, no-wrap
+#, fuzzy, no-wrap
+#| msgid ""
+#| "<div class=\"container\">\n"
+#| "  <div id=\"slides\">\n"
+#| "    <a href=\"Publications/Purkinje-2014\">\n"
+#| "      <img style=\"width:100%\" src=\"images/CAGEscan.png\" alt=\"Exploring the non-Coding Transcriptome with CAGEscan\">\n"
+#| "    </a>\n"
+#| "      <img style=\"width:100%\" src=\"images/logo.png\" alt=\"Logo\">\n"
+#| "    <a href=\"Publications/SL-CAGE-2014\">\n"
+#| "      <img style=\"width:100%\" src=\"images/SL-CAGE.png\" alt=\"Single-locus CAGE\">\n"
+#| "    </a>\n"
+#| "    <a href=\"Publications/LNA-2013\">\n"
+#| "      <img style=\"width:100%\" src=\"images/LNA.png\" alt=\"Template-Switching with Locked Nucleic Acids\">\n"
+#| "    </a>\n"
+#| "  </div>\n"
+#| "</div>\n"
 msgid ""
 "<div class=\"container\">\n"
 "  <div id=\"slides\">\n"
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+"    </a>\n"
+"      <img style=\"width:100%\" src=\"images/logo.png\" alt=\"Logo\">\n"
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Announce Pseudo-random primers on the front page.
diff --git a/index.mdwn b/index.mdwn
index 5e2dd58..82501db 100644
--- a/index.mdwn
+++ b/index.mdwn
@@ -24,10 +24,13 @@
 
 <div class="container">
   <div id="slides">
+    <a href="Publications/PseudoRandom-2016">
+      <img style="width:100%" src="images/PseudoRandomPrimers.png" alt="Pseudo-random primers">
+    </a>
+      <img style="width:100%" src="images/logo.png" alt="Logo">
     <a href="Publications/Purkinje-2014">
       <img style="width:100%" src="images/CAGEscan.png" alt="Exploring the non-Coding Transcriptome with CAGEscan">
     </a>
-      <img style="width:100%" src="images/logo.png" alt="Logo">
     <a href="Publications/SL-CAGE-2014">
       <img style="width:100%" src="images/SL-CAGE.png" alt="Single-locus CAGE">
     </a>

Pseud-random primers.
diff --git a/images/PseudoRandomPrimers.png b/images/PseudoRandomPrimers.png
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(Diff truncated)
updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index af79068..f371247 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
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 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
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@@ -241,6 +241,26 @@ msgid ""
 "explained in the [Biostars](https://www.biostars.org/p/170234/) forum)."
 msgstr ""
 
+#. type: Title ###
+#, no-wrap
+msgid "<a name=\"BWA\">Why are you still using BWA aln ?</a>"
+msgstr ""
+
+#. type: Plain text
+msgid ""
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+"bases reads.  In both cases, Read 1 is short (around 30 bases) and in our "
+"hands, the `aln` algorithm of [BWA](http://bio-bwa.sourceforge.net/) is a "
+"good enough tool to align it.  The length of Read 2 varies more according to "
+"the sequencer type.  On MiSeq it is around 20 bases, so `bwa aln` is still a "
+"good option.  On HiSeq, it is 50 bases, and arguably this causes more "
+"alignment failures because the probability to overlap a splice junction is "
+"higher.  Please let us know if you have a paired-end alignment strategy that "
+"would be equally efficient for the shortness of Read 1 and the relative "
+"longness of Read 2 on HiSeq, while still guaranteeing that there are no "
+"spurious gaps introduced at the 5′ end of Read 1."
+msgstr ""
+
 #. type: Title -
 #, no-wrap
 msgid "Bibliography\n"

Alignment with BWA.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 59a5107..68d6828 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -140,6 +140,21 @@ nanoCAGE (after corrections to the reference spike sequences explained in the
 [Biostars](https://www.biostars.org/p/170234/) forum).
 
 
+### <a name="BWA">Why are you still using BWA aln ?</a>
+
+We either sequence on MiSeq with the v2 50 cycles kit, or on HiSeq with
+50-bases reads.  In both cases, Read 1 is short (around 30 bases) and in our
+hands, the `aln` algorithm of [BWA](http://bio-bwa.sourceforge.net/) is a good
+enough tool to align it.  The length of Read 2 varies more according to the
+sequencer type.  On MiSeq it is around 20 bases, so `bwa aln` is still a good
+option.  On HiSeq, it is 50 bases, and arguably this causes more alignment
+failures because the probability to overlap a splice junction is higher.
+Please let us know if you have a paired-end alignment strategy that would be
+equally efficient for the shortness of Read 1 and the relative longness of Read
+2 on HiSeq, while still guaranteeing that there are no spurious gaps introduced
+at the 5′ end of Read 1.
+
+
 Bibliography
 ------------
 

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index d4ae8bb..af79068 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-02-18 05:39+0000\n"
+"POT-Creation-Date: 2016-02-18 05:40+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -230,7 +230,7 @@ msgstr ""
 #. type: Plain text
 msgid ""
 "_nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy "
-"_et al._, 2010](https://pubmed.gov/20543846) and others have shown that "
+"_et al._, 2010](https://pubmed.gov/20543846)) and others have shown that "
 "template switching is facilitated by the presence of a cap, but on the other "
 "hand it definitely works on non-capped molecules as well.  For that reason, "
 "the fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE "

Missing parenthesis.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 223ae85..59a5107 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -129,7 +129,7 @@ other RNA preparations, such as from ribosome pulldown ([Kratz _et al._,
 ### <a name="Spikes">Spikes are not capped.  Why can we use spikes ?</a>
 
 _nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy _et
-al._, 2010](https://pubmed.gov/20543846) and others have shown that template
+al._, 2010](https://pubmed.gov/20543846)) and others have shown that template
 switching is facilitated by the presence of a cap, but on the other hand it
 definitely works on non-capped molecules as well.  For that reason, the
 fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE than in

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index a3cf4f3..d4ae8bb 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2016-02-18 05:38+0000\n"
+"POT-Creation-Date: 2016-02-18 05:39+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -230,7 +230,7 @@ msgstr ""
 #. type: Plain text
 msgid ""
 "_nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy "
-"_et al., 2010](https://pubmed.gov/20543846) and others have shown that "
+"_et al._, 2010](https://pubmed.gov/20543846) and others have shown that "
 "template switching is facilitated by the presence of a cap, but on the other "
 "hand it definitely works on non-capped molecules as well.  For that reason, "
 "the fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE "

Typo.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index edf54a0..223ae85 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -129,7 +129,7 @@ other RNA preparations, such as from ribosome pulldown ([Kratz _et al._,
 ### <a name="Spikes">Spikes are not capped.  Why can we use spikes ?</a>
 
 _nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy _et
-al., 2010](https://pubmed.gov/20543846) and others have shown that template
+al._, 2010](https://pubmed.gov/20543846) and others have shown that template
 switching is facilitated by the presence of a cap, but on the other hand it
 definitely works on non-capped molecules as well.  For that reason, the
 fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE than in

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index 6881199..a3cf4f3 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-07-01 08:57+0000\n"
+"POT-Creation-Date: 2016-02-18 05:38+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -222,6 +222,25 @@ msgid ""
 "pulldown ([Kratz _et al._, 2014](http://dx.doi.org/10.1101/gr.164095.113))."
 msgstr ""
 
+#. type: Title ###
+#, no-wrap
+msgid "<a name=\"Spikes\">Spikes are not capped.  Why can we use spikes ?</a>"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"_nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy "
+"_et al., 2010](https://pubmed.gov/20543846) and others have shown that "
+"template switching is facilitated by the presence of a cap, but on the other "
+"hand it definitely works on non-capped molecules as well.  For that reason, "
+"the fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE "
+"than in the more stringent CAGE protocols based on the chemical _[CAP "
+"Trapper](https://pubmed.gov/8938445)_ method, used for instance in the "
+"FANTOM and ENCODE projects.  Therefore, we can detect the non-capped ERCC "
+"spikes in nanoCAGE (after corrections to the reference spike sequences "
+"explained in the [Biostars](https://www.biostars.org/p/170234/) forum)."
+msgstr ""
+
 #. type: Title -
 #, no-wrap
 msgid "Bibliography\n"

FAQ on spikes.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 354d8cd..edf54a0 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -126,6 +126,20 @@ other RNA preparations, such as from ribosome pulldown ([Kratz _et al._,
 2014](http://dx.doi.org/10.1101/gr.164095.113)). 
 
 
+### <a name="Spikes">Spikes are not capped.  Why can we use spikes ?</a>
+
+_nanoCAGE_ uses template switching to add linkers at 5′ ends.  We ([Plessy _et
+al., 2010](https://pubmed.gov/20543846) and others have shown that template
+switching is facilitated by the presence of a cap, but on the other hand it
+definitely works on non-capped molecules as well.  For that reason, the
+fraction of reads aligning to the ribosomal RNAs is higher in nanoCAGE than in
+the more stringent CAGE protocols based on the chemical _[CAP
+Trapper](https://pubmed.gov/8938445)_ method, used for instance in the FANTOM
+and ENCODE projects.  Therefore, we can detect the non-capped ERCC spikes in
+nanoCAGE (after corrections to the reference spike sequences explained in the
+[Biostars](https://www.biostars.org/p/170234/) forum).
+
+
 Bibliography
 ------------
 

updated PO files
diff --git a/News/2014/KI-ncRNA-2014.ja.po b/News/2014/KI-ncRNA-2014.ja.po
index 80a74eb..c55e828 100644
--- a/News/2014/KI-ncRNA-2014.ja.po
+++ b/News/2014/KI-ncRNA-2014.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2014-03-03 04:34+0000\n"
+"POT-Creation-Date: 2015-12-02 12:37+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -37,7 +37,7 @@ msgid ""
 "On February 25<sup>th</sup> [[People/Charles_Plessy]] gave a lecture on\n"
 "_non-translated RNAs_ at the Karolinska Institutet - RIKEN [[Joint\n"
 "International Doctoral\n"
-"Course|http://www.clst.riken.jp/en/event/2014/riken-ki.html]] 2014 on\n"
+"Course|http://kiwas.ki.se/katalog/katalog/kurs/1685?lang=en]] 2014 on\n"
 "_Employing genome-wide technologies for functional regulation in development\n"
 "and disease_, in Stockholm.\n"
 msgstr ""

Replace borken link.
diff --git a/News/2014/KI-ncRNA-2014.mdwn b/News/2014/KI-ncRNA-2014.mdwn
index 6e2dc59..7ccbe55 100644
--- a/News/2014/KI-ncRNA-2014.mdwn
+++ b/News/2014/KI-ncRNA-2014.mdwn
@@ -5,6 +5,6 @@
 On February 25<sup>th</sup> [[People/Charles_Plessy]] gave a lecture on
 _non-translated RNAs_ at the Karolinska Institutet - RIKEN [[Joint
 International Doctoral
-Course|http://www.clst.riken.jp/en/event/2014/riken-ki.html]] 2014 on
+Course|http://kiwas.ki.se/katalog/katalog/kurs/1685?lang=en]] 2014 on
 _Employing genome-wide technologies for functional regulation in development
 and disease_, in Stockholm.

updated PO files
diff --git a/News/2015/IMGC_2015.ja.po b/News/2015/IMGC_2015.ja.po
new file mode 100644
index 0000000..cfc228f
--- /dev/null
+++ b/News/2015/IMGC_2015.ja.po
@@ -0,0 +1,42 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-11-17 05:33+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Talk at the IMGC 2015 on CAGEscan fragments.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"09 Nov 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Meeting People/Charles_Plessy]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"On November 9<sup>th</sup> [[People/Charles_Plessy]] gave a talk\n"
+"on [_Single-molecule RNA sequencing in single "
+"cells_](http://imgc2015.jp/abstracts.html#O-12)\n"
+"at the International Mammalian Genomics Conference \n"
+"([IMGC](http://imgc2015.jp/))in Yokohama, Japan.\n"
+msgstr ""

IMGC 2015
diff --git a/News/2015/IMGC_2015.mdwn b/News/2015/IMGC_2015.mdwn
new file mode 100644
index 0000000..6a6d1e1
--- /dev/null
+++ b/News/2015/IMGC_2015.mdwn
@@ -0,0 +1,8 @@
+[[!meta title="Talk at the IMGC 2015 on CAGEscan fragments."]]
+[[!meta date="09 Nov 2015"]]
+[[!tag Meeting People/Charles_Plessy]]
+
+On November 9<sup>th</sup> [[People/Charles_Plessy]] gave a talk
+on [_Single-molecule RNA sequencing in single cells_](http://imgc2015.jp/abstracts.html#O-12)
+at the International Mammalian Genomics Conference 
+([IMGC](http://imgc2015.jp/))in Yokohama, Japan.

Unfuzzy.
diff --git a/People/Charles_Plessy.ja.po b/People/Charles_Plessy.ja.po
index 7bcb945..7300c69 100644
--- a/People/Charles_Plessy.ja.po
+++ b/People/Charles_Plessy.ja.po
@@ -82,18 +82,6 @@ msgstr ""
 "FANTOMコンソーシアムのメンバーとしてもCAGE法の開発と分析に貢献しています。"
 
 #. type: Plain text
-#, fuzzy
-#| msgid ""
-#| "Together with my colleagues at RIKEN and collaborators in the field of "
-#| "neuroscience, I have applied nanoCAGE to the study of single neuron cell "
-#| "types, for instance the olfactory neurons ([Plessy et al., 2012](http://"
-#| "pubmed.org/22194471)), or in dopaminergic cells, where we could "
-#| "demonstrate the expression of haemoglobin in the midbrain ([Biagioli et "
-#| "al., 2009](http://pubmed.org/19717439)).  We are also exploring the sub-"
-#| "cellular localisation of RNA in Purkinje neurons ([Kratz et al, 2014]"
-#| "(http://pubmed.org/24904046)).  In parallel with this promoter-centric "
-#| "work, I am also exploring the huge [repertoire](http://clonotyper."
-#| "brancable.com) of the T cell antigen receptors."
 msgid ""
 "Together with my colleagues at RIKEN and collaborators in the field of "
 "neuroscience, I have applied nanoCAGE to the study of single neuron cell "

updated PO files
diff --git a/People/Charles_Plessy.ja.po b/People/Charles_Plessy.ja.po
index d71292b..7bcb945 100644
--- a/People/Charles_Plessy.ja.po
+++ b/People/Charles_Plessy.ja.po
@@ -1,7 +1,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2015-07-02 06:45+0000\n"
+"POT-Creation-Date: 2015-11-17 05:26+0000\n"
 "PO-Revision-Date: 2014-07-07 20:05+0900\n"
 "Last-Translator: Charles Plessy <plessy@riken.jp>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -82,6 +82,18 @@ msgstr ""
 "FANTOMコンソーシアムのメンバーとしてもCAGE法の開発と分析に貢献しています。"
 
 #. type: Plain text
+#, fuzzy
+#| msgid ""
+#| "Together with my colleagues at RIKEN and collaborators in the field of "
+#| "neuroscience, I have applied nanoCAGE to the study of single neuron cell "
+#| "types, for instance the olfactory neurons ([Plessy et al., 2012](http://"
+#| "pubmed.org/22194471)), or in dopaminergic cells, where we could "
+#| "demonstrate the expression of haemoglobin in the midbrain ([Biagioli et "
+#| "al., 2009](http://pubmed.org/19717439)).  We are also exploring the sub-"
+#| "cellular localisation of RNA in Purkinje neurons ([Kratz et al, 2014]"
+#| "(http://pubmed.org/24904046)).  In parallel with this promoter-centric "
+#| "work, I am also exploring the huge [repertoire](http://clonotyper."
+#| "brancable.com) of the T cell antigen receptors."
 msgid ""
 "Together with my colleagues at RIKEN and collaborators in the field of "
 "neuroscience, I have applied nanoCAGE to the study of single neuron cell "
@@ -91,7 +103,7 @@ msgid ""
 "(http://pubmed.org/19717439)).  We are also exploring the sub-cellular "
 "localisation of RNA in Purkinje neurons ([Kratz et al, 2014](http://pubmed."
 "org/24904046)).  In parallel with this promoter-centric work, I am also "
-"exploring the huge [repertoire](http://clonotyper.brancable.com) of the T "
+"exploring the huge [repertoire](http://clonotyper.branchable.com) of the T "
 "cell antigen receptors."
 msgstr ""
 "一方、nanoCAGE法を単一神経細胞タイプの研究に応用し、理化学研究所および脳科学"

Typo in URL.
diff --git a/People/Charles_Plessy.mdwn b/People/Charles_Plessy.mdwn
index c767940..2148764 100644
--- a/People/Charles_Plessy.mdwn
+++ b/People/Charles_Plessy.mdwn
@@ -40,7 +40,7 @@ demonstrate the expression of haemoglobin in the midbrain ([Biagioli et al.,
 2009](http://pubmed.org/19717439)).  We are also exploring the sub-cellular
 localisation of RNA in Purkinje neurons ([Kratz et al, 2014](http://pubmed.org/24904046)).
 In parallel with this promoter-centric work, I am also exploring the huge
-[repertoire](http://clonotyper.brancable.com) of the T cell antigen receptors.
+[repertoire](http://clonotyper.branchable.com) of the T cell antigen receptors.
 
 I am also a Free Software enthusiast, and contribute to the [Debian
 Med](http://www.debian.org/devel/debian-med) project, by

updated PO files
diff --git a/News/2015/C1_CAGE.ja.po b/News/2015/C1_CAGE.ja.po
new file mode 100644
index 0000000..11df519
--- /dev/null
+++ b/News/2015/C1_CAGE.ja.po
@@ -0,0 +1,47 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-10-16 03:40+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"C1 CAGE released.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"15 oct 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag pseudo-random people/Charles_Plessy]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Today, our single-cell CAGE protocol for the Fluidigm C1 system was released "
+"on the maker's [ScriptHub][] page.  Of course, we also plan to release this "
+"work as a peer-reviewed article later.  A pilot analysis of preview data is "
+"also available on [GitHub][]."
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"[ScriptHub]: "
+"https://www.fluidigm.com/c1openapp/scripthub/script/2015-07/c1-cage-1436761405138-3 "
+"[GitHub]: https://github.com/Population-Transcriptomics/C1-CAGE-preview"
+msgstr ""

C1 CAGE !
diff --git a/News/2015/C1_CAGE.mdwn b/News/2015/C1_CAGE.mdwn
new file mode 100644
index 0000000..c01021c
--- /dev/null
+++ b/News/2015/C1_CAGE.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="C1 CAGE released."]]
+[[!meta date="15 oct 2015"]]
+[[!tag pseudo-random people/Charles_Plessy]]
+
+Today, our single-cell CAGE protocol for the Fluidigm C1 system was released on
+the maker's [ScriptHub][] page.  Of course, we also plan to release this work
+as a peer-reviewed article later.  A pilot analysis of preview data is also
+available on [GitHub][].
+
+[ScriptHub]: https://www.fluidigm.com/c1openapp/scripthub/script/2015-07/c1-cage-1436761405138-3
+[GitHub]: https://github.com/Population-Transcriptomics/C1-CAGE-preview

The FANTOM3 CAGE paper.
diff --git a/bibliography/16645617.mdwn b/bibliography/16645617.mdwn
new file mode 100644
index 0000000..c58bbdd
--- /dev/null
+++ b/bibliography/16645617.mdwn
@@ -0,0 +1,7 @@
+[[!tag bibliography People/Charles_Plessy]]
+[[!meta date="01 Jun 2006"]]
+
+Carninci P, Sandelin A, Lenhard B, Katayama S, Shimokawa K, Ponjavic J, Semple CA, Taylor MS, Engström PG, Frith MC, Forrest AR, Alkema WB, Tan SL, Plessy C, Kodzius R, Ravasi T, Kasukawa T, Fukuda S, Kanamori-Katayama M, Kitazume Y, Kawaji H, Kai C, Nakamura M, Konno H, Nakano K, Mottagui-Tabar S, Arner P, Chesi A, Gustincich S, Persichetti F, Suzuki H, Grimmond SM, Wells CA, Orlando V, Wahlestedt C, Liu ET, Harbers M, Kawai J, Bajic VB, Hume DA, Hayashizaki Y.
+Genome-wide analysis of mammalian promoter architecture and evolution.
+[[!doi 10.1038/ng1789 desc="Nat Genet. **2006** Jun;38(6):626-35."]]
+[[!PMID 16645617]]

Use the print publication date.
diff --git a/bibliography/25505068.mdwn b/bibliography/25505068.mdwn
index 2ddd69b..72b5a51 100644
--- a/bibliography/25505068.mdwn
+++ b/bibliography/25505068.mdwn
@@ -1,7 +1,7 @@
 [[!tag bibliography People/Charles_Plessy]]
-[[!meta date="10 Dec 2014"]]
+[[!meta date="01 Feb 2015"]]
 
 Taguchi A, Nagasaka K<sup>✉</sup>, Kawana K<sup>✉</sup>, Hashimoto K, Kusumoto-Matsuo R, Plessy C, Thomas M, Nakamura H, Bonetti A, Oda K, Kukimoto I, Carninci P, Banks L, Osuga Y, Fujii T.
 _Characterization of novel transcripts of human papillomavirus type 16 using CAGE technology._
-[[!doi 10.1128/JVI.03433-14 desc="J Virol. **2014** Dec 10. pii: JVI.03433-14"]]
+[[!doi 10.1128/JVI.03433-14 desc="J Virol. **2015**  Feb;89(4):2448-52"]]
 [[!PMID 25505068]]

updated PO files
diff --git a/News/2015/pseudo-random.ja.po b/News/2015/pseudo-random.ja.po
new file mode 100644
index 0000000..842e881
--- /dev/null
+++ b/News/2015/pseudo-random.ja.po
@@ -0,0 +1,39 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-09-30 06:06+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Learn more about pseudo-random primers on bioRxiv.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"29 sep 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag pseudo-random people/Charles_Plessy]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"Our work on _pseudo-random_ primers is completed, and our manuscript is "
+"submitted to a peer-reviewed journal.  You can already read our preprint on "
+"[bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805)."
+msgstr ""

Pseudo-randoms on bioRxiv.
diff --git a/News/2015/pseudo-random.mdwn b/News/2015/pseudo-random.mdwn
new file mode 100644
index 0000000..da5b544
--- /dev/null
+++ b/News/2015/pseudo-random.mdwn
@@ -0,0 +1,7 @@
+[[!meta title="Learn more about pseudo-random primers on bioRxiv."]]
+[[!meta date="29 sep 2015"]]
+[[!tag pseudo-random people/Charles_Plessy]]
+
+Our work on _pseudo-random_ primers is completed, and our manuscript is
+submitted to a peer-reviewed journal.  You can already read our preprint
+on [bioRxiv](http://www.biorxiv.org/content/early/2015/09/29/027805).

updated PO files
diff --git a/News/2015/SGC_2015.ja.po b/News/2015/SGC_2015.ja.po
index 9ed22ab..b7b8c58 100644
--- a/News/2015/SGC_2015.ja.po
+++ b/News/2015/SGC_2015.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-09-25 00:54+0000\n"
+"POT-Creation-Date: 2015-09-25 00:55+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -40,7 +40,7 @@ msgid ""
 "cells_ and _Cell-cycle classification at the single-cell level with Random "
 "Forest_ respectively.  Here is a link to the [abstract book](http://www."
 "weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/"
-"scg_2015_abstractbook_online_version_0.pdf)  in PDF format (poster P008 and "
+"scg_2015_abstractbook_online_version_0.pdf)  in PDF format (posters P008 and "
 "P058).  These works are related to the [[data|cell-cycle-on-github]] that we "
 "released in DDBJ, GitHub and Dryad recently."
 msgstr ""

Typo.
diff --git a/News/2015/SGC_2015.mdwn b/News/2015/SGC_2015.mdwn
index b6370c4..301f7a3 100644
--- a/News/2015/SGC_2015.mdwn
+++ b/News/2015/SGC_2015.mdwn
@@ -8,6 +8,6 @@ Mickaël Mendez presented our work on _Single-cell transcriptomes of
 fluorescent, ubiquitination-based cell cycle indicator cells_ and _Cell-cycle
 classification at the single-cell level with Random Forest_ respectively.  Here
 is a link to the [abstract book](http://www.weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/scg_2015_abstractbook_online_version_0.pdf)
-in PDF format (poster P008 and P058).  These works are related to the
+in PDF format (posters P008 and P058).  These works are related to the
 [[data|cell-cycle-on-github]] that we released in DDBJ, GitHub and Dryad
 recently. 

updated PO files
diff --git a/News/2015/SGC_2015.ja.po b/News/2015/SGC_2015.ja.po
index 3c451ab..9ed22ab 100644
--- a/News/2015/SGC_2015.ja.po
+++ b/News/2015/SGC_2015.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-09-25 00:53+0000\n"
+"POT-Creation-Date: 2015-09-25 00:54+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -18,9 +18,7 @@ msgstr ""
 
 #. type: Plain text
 #, no-wrap
-msgid ""
-"[[!meta title=\"Posters presented at the Single Cell Genomics conference "
-"2015.\"]]\n"
+msgid "[[!meta title=\"Posters presented at the Single Cell Genomics conference 2015.\"]]\n"
 msgstr ""
 
 #. type: Plain text
@@ -35,15 +33,14 @@ msgstr ""
 
 #. type: Plain text
 msgid ""
-"At the [Single Cell Genomics "
-"conference](http://www.weizmann.ac.il/conferences/SCG2015)  that took place "
-"in Utrecht from September 16 to 18, Michael Böttcher and Mickaël Mendez "
-"presented our work on _Single-cell transcriptomes of fluorescent, "
-"ubiquitination-based cell cycle indicator cells_ and _Cell-cycle "
-"classification at the single-cell level with Random Forest_ respectively.  "
-"Here is a link to the [abstract "
-"book](http://www.weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/scg_2015_abstractbook_online_version_0.pdf)  "
-"in PDF format (poster P008 and P058).  These works are related to the "
-"[[cell-cycle-on-github|data]] that we released in DDBJ, GitHub and Dryad "
-"recently."
+"At the [Single Cell Genomics conference](http://www.weizmann.ac.il/"
+"conferences/SCG2015)  that took place in Utrecht from September 16 to 18, "
+"Michael Böttcher and Mickaël Mendez presented our work on _Single-cell "
+"transcriptomes of fluorescent, ubiquitination-based cell cycle indicator "
+"cells_ and _Cell-cycle classification at the single-cell level with Random "
+"Forest_ respectively.  Here is a link to the [abstract book](http://www."
+"weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/"
+"scg_2015_abstractbook_online_version_0.pdf)  in PDF format (poster P008 and "
+"P058).  These works are related to the [[data|cell-cycle-on-github]] that we "
+"released in DDBJ, GitHub and Dryad recently."
 msgstr ""

Syntax correction.
diff --git a/News/2015/SGC_2015.mdwn b/News/2015/SGC_2015.mdwn
index 954a20f..b6370c4 100644
--- a/News/2015/SGC_2015.mdwn
+++ b/News/2015/SGC_2015.mdwn
@@ -9,5 +9,5 @@ fluorescent, ubiquitination-based cell cycle indicator cells_ and _Cell-cycle
 classification at the single-cell level with Random Forest_ respectively.  Here
 is a link to the [abstract book](http://www.weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/scg_2015_abstractbook_online_version_0.pdf)
 in PDF format (poster P008 and P058).  These works are related to the
-[[cell-cycle-on-github|data]] that we released in DDBJ, GitHub and Dryad
+[[data|cell-cycle-on-github]] that we released in DDBJ, GitHub and Dryad
 recently. 

updated PO files
diff --git a/News/2015/SGC_2015.ja.po b/News/2015/SGC_2015.ja.po
new file mode 100644
index 0000000..3c451ab
--- /dev/null
+++ b/News/2015/SGC_2015.ja.po
@@ -0,0 +1,49 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-09-25 00:53+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!meta title=\"Posters presented at the Single Cell Genomics conference "
+"2015.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"18 sep 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag cell_cycle conference]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"At the [Single Cell Genomics "
+"conference](http://www.weizmann.ac.il/conferences/SCG2015)  that took place "
+"in Utrecht from September 16 to 18, Michael Böttcher and Mickaël Mendez "
+"presented our work on _Single-cell transcriptomes of fluorescent, "
+"ubiquitination-based cell cycle indicator cells_ and _Cell-cycle "
+"classification at the single-cell level with Random Forest_ respectively.  "
+"Here is a link to the [abstract "
+"book](http://www.weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/scg_2015_abstractbook_online_version_0.pdf)  "
+"in PDF format (poster P008 and P058).  These works are related to the "
+"[[cell-cycle-on-github|data]] that we released in DDBJ, GitHub and Dryad "
+"recently."
+msgstr ""

Posters presented.
diff --git a/News/2015/SGC_2015.mdwn b/News/2015/SGC_2015.mdwn
new file mode 100644
index 0000000..954a20f
--- /dev/null
+++ b/News/2015/SGC_2015.mdwn
@@ -0,0 +1,13 @@
+[[!meta title="Posters presented at the Single Cell Genomics conference 2015."]]
+[[!meta date="18 sep 2015"]]
+[[!tag cell_cycle conference]]
+
+At the [Single Cell Genomics conference](http://www.weizmann.ac.il/conferences/SCG2015)
+that took place in Utrecht from September 16 to 18, Michael Böttcher and
+Mickaël Mendez presented our work on _Single-cell transcriptomes of
+fluorescent, ubiquitination-based cell cycle indicator cells_ and _Cell-cycle
+classification at the single-cell level with Random Forest_ respectively.  Here
+is a link to the [abstract book](http://www.weizmann.ac.il/conferences/SCG2015/sites/conferences.SCG2015/files/scg_2015_abstractbook_online_version_0.pdf)
+in PDF format (poster P008 and P058).  These works are related to the
+[[cell-cycle-on-github|data]] that we released in DDBJ, GitHub and Dryad
+recently. 

updated PO files
diff --git a/News/2015/pairedBamToBed12.ja.po b/News/2015/pairedBamToBed12.ja.po
new file mode 100644
index 0000000..5672076
--- /dev/null
+++ b/News/2015/pairedBamToBed12.ja.po
@@ -0,0 +1,44 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-07-31 03:48+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Paired-end conversion tool released on GitHub\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"30 jul 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag nanoCAGE GitHub]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"We released our tool called `pairedBamToBed12` on "
+"[GitHub](https://github.com/Population-Transcriptomics/pairedBamToBed12) "
+"today.  This tool converts paired-end data from the BAM to the BED12 format, "
+"and is especially useful to proces [[CAGEscan|nanoCAGE#CAGEscan]] data "
+"before displaying it on the [Zenbu](http://fantom.gsc.riken.jp/zenbu/) "
+"browser.  See the "
+"[documentation](https://github.com/Population-Transcriptomics/pairedBamToBed12#usage-with-transcriptome-libraries)  "
+"for more details."
+msgstr ""

Released pairedBamToBed12 on GitHub.
diff --git a/News/2015/pairedBamToBed12.mdwn b/News/2015/pairedBamToBed12.mdwn
new file mode 100644
index 0000000..a2846fe
--- /dev/null
+++ b/News/2015/pairedBamToBed12.mdwn
@@ -0,0 +1,11 @@
+[[!meta title="Paired-end conversion tool released on GitHub"]]
+[[!meta date="30 jul 2015"]]
+[[!tag nanoCAGE GitHub]]
+
+We released our tool called `pairedBamToBed12` on
+[GitHub](https://github.com/Population-Transcriptomics/pairedBamToBed12) today.
+This tool converts paired-end data from the BAM to the BED12 format, and is
+especially useful to proces [[CAGEscan|nanoCAGE#CAGEscan]] data before
+displaying it on the [Zenbu](http://fantom.gsc.riken.jp/zenbu/) browser.  See
+the [documentation](https://github.com/Population-Transcriptomics/pairedBamToBed12#usage-with-transcriptome-libraries)
+for more details.

updated PO files
diff --git a/News/2015/DNATV_2015.ja.po b/News/2015/DNATV_2015.ja.po
index 085bce0..0067fdf 100644
--- a/News/2015/DNATV_2015.ja.po
+++ b/News/2015/DNATV_2015.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-07-31 02:38+0000\n"
+"POT-Creation-Date: 2015-07-31 02:39+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -34,8 +34,7 @@ msgstr ""
 #. type: Plain text
 #, no-wrap
 msgid ""
-"On July 24<sup>th</sup> [[Charles_Plessy]] presented a poster on _methods\n"
+"On July 24<sup>th</sup> [[People/Charles_Plessy]] presented a poster on _methods\n"
 "for transcriptome analysis applied to the human papillomavirus_ at the\n"
-"[DNA Tumor Virus meeting 2015](http://www.icgeb.org/dnatv.html) in Trieste, "
-"Italy.\n"
+"[DNA Tumor Virus meeting 2015](http://www.icgeb.org/dnatv.html) in Trieste, Italy.\n"
 msgstr ""

Corrected syntax.
diff --git a/News/2015/DNATV_2015.mdwn b/News/2015/DNATV_2015.mdwn
index 0aa29c8..2122e24 100644
--- a/News/2015/DNATV_2015.mdwn
+++ b/News/2015/DNATV_2015.mdwn
@@ -2,6 +2,6 @@
 [[!meta date="24 Jul 2015"]]
 [[!tag Meeting]]
 
-On July 24<sup>th</sup> [[Charles_Plessy]] presented a poster on _methods
+On July 24<sup>th</sup> [[People/Charles_Plessy]] presented a poster on _methods
 for transcriptome analysis applied to the human papillomavirus_ at the
 [DNA Tumor Virus meeting 2015](http://www.icgeb.org/dnatv.html) in Trieste, Italy.

updated PO files
diff --git a/News/2015/DNATV_2015.ja.po b/News/2015/DNATV_2015.ja.po
new file mode 100644
index 0000000..085bce0
--- /dev/null
+++ b/News/2015/DNATV_2015.ja.po
@@ -0,0 +1,41 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-07-31 02:38+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Poster at the DNA Tumor Virus meeting 2015.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"24 Jul 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Meeting]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"On July 24<sup>th</sup> [[Charles_Plessy]] presented a poster on _methods\n"
+"for transcriptome analysis applied to the human papillomavirus_ at the\n"
+"[DNA Tumor Virus meeting 2015](http://www.icgeb.org/dnatv.html) in Trieste, "
+"Italy.\n"
+msgstr ""

Presented poster.
diff --git a/News/2015/DNATV_2015.mdwn b/News/2015/DNATV_2015.mdwn
new file mode 100644
index 0000000..0aa29c8
--- /dev/null
+++ b/News/2015/DNATV_2015.mdwn
@@ -0,0 +1,7 @@
+[[!meta title="Poster at the DNA Tumor Virus meeting 2015."]]
+[[!meta date="24 Jul 2015"]]
+[[!tag Meeting]]
+
+On July 24<sup>th</sup> [[Charles_Plessy]] presented a poster on _methods
+for transcriptome analysis applied to the human papillomavirus_ at the
+[DNA Tumor Virus meeting 2015](http://www.icgeb.org/dnatv.html) in Trieste, Italy.

updated PO files
diff --git a/News/2015/cell-cycle-on-github.ja.po b/News/2015/cell-cycle-on-github.ja.po
new file mode 100644
index 0000000..fff0a92
--- /dev/null
+++ b/News/2015/cell-cycle-on-github.ja.po
@@ -0,0 +1,43 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-07-15 06:09+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Single-cell cell cycle metadata released on GitHub\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"15 jul 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag cell_cycle]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"In order to classify single-cell transcriptomes by cell cycle phases, we "
+"produced a reference data set comprising more than 200 single-cell RNA-seq "
+"libraries, together with cell cycle information for each cell.  While our "
+"analysis is ongoing, we are releasing prior publication some documentation "
+"on this data on "
+"[GitHub](https://github.com/Population-Transcriptomics/Cell-Cycle-on-C1).  "
+"Pull requests are welcome !"
+msgstr ""

Advertise our data release.
diff --git a/News/2015/cell-cycle-on-github.mdwn b/News/2015/cell-cycle-on-github.mdwn
new file mode 100644
index 0000000..c1cee40
--- /dev/null
+++ b/News/2015/cell-cycle-on-github.mdwn
@@ -0,0 +1,10 @@
+[[!meta title="Single-cell cell cycle metadata released on GitHub"]]
+[[!meta date="15 jul 2015"]]
+[[!tag cell_cycle]]
+
+In order to classify single-cell transcriptomes by cell cycle phases, we
+produced a reference data set comprising more than 200 single-cell RNA-seq
+libraries, together with cell cycle information for each cell.  While our
+analysis is ongoing, we are releasing prior publication some documentation on
+this data on [GitHub](https://github.com/Population-Transcriptomics/Cell-Cycle-on-C1).
+Pull requests are welcome !

updated PO files
diff --git a/People/Charles_Plessy.ja.po b/People/Charles_Plessy.ja.po
index 254379c..d71292b 100644
--- a/People/Charles_Plessy.ja.po
+++ b/People/Charles_Plessy.ja.po
@@ -1,7 +1,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2015-07-02 06:44+0000\n"
+"POT-Creation-Date: 2015-07-02 06:45+0000\n"
 "PO-Revision-Date: 2014-07-07 20:05+0900\n"
 "Last-Translator: Charles Plessy <plessy@riken.jp>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -134,7 +134,7 @@ msgstr ""
 
 #. type: Plain text
 msgid ""
-"My ORCID ID is [http://orcid.org/0000-0001-7410-6295](0000-0001-7410-6295)."
+"My ORCID ID is [0000-0001-7410-6295](http://orcid.org/0000-0001-7410-6295)."
 msgstr ""
 
 #. type: Title -

Repair borken link.
diff --git a/People/Charles_Plessy.mdwn b/People/Charles_Plessy.mdwn
index 0a51911..c767940 100644
--- a/People/Charles_Plessy.mdwn
+++ b/People/Charles_Plessy.mdwn
@@ -57,7 +57,7 @@ Genomics Technology, to expand this work on single cells following a population
 transcriptomics approach ([Plessy et al., 2013](https://www.ncbi.nlm.nih.gov/pubmed/23281054))
 focused on sampling the largest possible number of cells.
 
-My ORCID ID is [http://orcid.org/0000-0001-7410-6295](0000-0001-7410-6295).
+My ORCID ID is [0000-0001-7410-6295](http://orcid.org/0000-0001-7410-6295).
 
 Recent news
 -----------

updated PO files
diff --git a/People/Charles_Plessy.ja.po b/People/Charles_Plessy.ja.po
index 0094716..254379c 100644
--- a/People/Charles_Plessy.ja.po
+++ b/People/Charles_Plessy.ja.po
@@ -1,7 +1,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: \n"
-"POT-Creation-Date: 2014-07-07 11:04+0000\n"
+"POT-Creation-Date: 2015-07-02 06:44+0000\n"
 "PO-Revision-Date: 2014-07-07 20:05+0900\n"
 "Last-Translator: Charles Plessy <plessy@riken.jp>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -99,8 +99,8 @@ msgstr ""
 "org/22194471))やドーパミン作動性細胞([Biagioli et al., 2009](http://pubmed."
 "org/19717439))の研究を実施。ドーパミン作動性細胞の研究では、中脳におけるヘモ"
 "グロビンの発現を証明しました。さらにプルキンエ神経細胞におけるRNAの細胞内局在"
-"性を探究([Kratz et al, 2014](http://pubmed.org/24904046))。プロモーター中心"
-"の同研究と並行して、T細胞抗原受容体の大規模[レパートリー](http://clonotyper."
+"性を探究([Kratz et al, 2014](http://pubmed.org/24904046))。プロモーター中心の"
+"同研究と並行して、T細胞抗原受容体の大規模[レパートリー](http://clonotyper."
 "branchable.com)の研究も行っています。"
 
 #. type: Plain text
@@ -132,6 +132,11 @@ msgstr ""
 "量技術開発ユニットのリーダーとして、2013年4月、新たな開発サイクルを開始しまし"
 "た。"
 
+#. type: Plain text
+msgid ""
+"My ORCID ID is [http://orcid.org/0000-0001-7410-6295](0000-0001-7410-6295)."
+msgstr ""
+
 #. type: Title -
 #, no-wrap
 msgid "Recent news\n"

Link to ORCID ID.
diff --git a/People/Charles_Plessy.mdwn b/People/Charles_Plessy.mdwn
index bcc7f11..0a51911 100644
--- a/People/Charles_Plessy.mdwn
+++ b/People/Charles_Plessy.mdwn
@@ -57,6 +57,8 @@ Genomics Technology, to expand this work on single cells following a population
 transcriptomics approach ([Plessy et al., 2013](https://www.ncbi.nlm.nih.gov/pubmed/23281054))
 focused on sampling the largest possible number of cells.
 
+My ORCID ID is [http://orcid.org/0000-0001-7410-6295](0000-0001-7410-6295).
+
 Recent news
 -----------
 

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index 9381be8..6881199 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-07-01 08:55+0000\n"
+"POT-Creation-Date: 2015-07-01 08:57+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -215,10 +215,10 @@ msgstr ""
 msgid ""
 "_nanoCAGE_ works on total RNAs from a broad range of species.  We used it "
 "mostly on vertebrate RNAs, but there is also a published report using insect "
-"total RNA (Honey Bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/"
+"total RNA (honey bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/"
 "srep11136)).  We also produced libraries from plant (_Arabidopsis_), Yeast "
 "(_S. pombe_) and bacterial (_E. coli_) total RNA (unpublished results). "
-"_nanoCAGE_ also work on other RNA preparations, such as from ribosome "
+"_nanoCAGE_ also works on other RNA preparations, such as from ribosome "
 "pulldown ([Kratz _et al._, 2014](http://dx.doi.org/10.1101/gr.164095.113))."
 msgstr ""
 

Proofreading.
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index f37b426..354d8cd 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -119,9 +119,9 @@ cDNAs are not cleaved in small tags, for instance in the nAnTi-CAGE protocol
 
 _nanoCAGE_ works on total RNAs from a broad range of species.  We used it
 mostly on vertebrate RNAs, but there is also a published report using insect
-total RNA (Honey Bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/srep11136)).
+total RNA (honey bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/srep11136)).
 We also produced libraries from plant (_Arabidopsis_), Yeast (_S. pombe_) and
-bacterial (_E. coli_) total RNA (unpublished results). _nanoCAGE_ also work on
+bacterial (_E. coli_) total RNA (unpublished results). _nanoCAGE_ also works on
 other RNA preparations, such as from ribosome pulldown ([Kratz _et al._,
 2014](http://dx.doi.org/10.1101/gr.164095.113)). 
 

updated PO files
diff --git a/nanoCAGE.ja.po b/nanoCAGE.ja.po
index f9637b7..9381be8 100644
--- a/nanoCAGE.ja.po
+++ b/nanoCAGE.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2014-11-18 00:47+0000\n"
+"POT-Creation-Date: 2015-07-01 08:55+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -206,6 +206,22 @@ msgid ""
 "([Murata _et al._, 2014](http://dx.doi.org/10.1007%2F978-1-4939-0805-9_7))."
 msgstr ""
 
+#. type: Title ###
+#, no-wrap
+msgid "<a name=\"RNA_origin\">On what RNAs does it work ?</a>"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"_nanoCAGE_ works on total RNAs from a broad range of species.  We used it "
+"mostly on vertebrate RNAs, but there is also a published report using insect "
+"total RNA (Honey Bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/"
+"srep11136)).  We also produced libraries from plant (_Arabidopsis_), Yeast "
+"(_S. pombe_) and bacterial (_E. coli_) total RNA (unpublished results). "
+"_nanoCAGE_ also work on other RNA preparations, such as from ribosome "
+"pulldown ([Kratz _et al._, 2014](http://dx.doi.org/10.1101/gr.164095.113))."
+msgstr ""
+
 #. type: Title -
 #, no-wrap
 msgid "Bibliography\n"

On what RNAs does nanoCAGE work ?
diff --git a/nanoCAGE.mdwn b/nanoCAGE.mdwn
index 44344c7..f37b426 100644
--- a/nanoCAGE.mdwn
+++ b/nanoCAGE.mdwn
@@ -115,6 +115,17 @@ cDNAs are not cleaved in small tags, for instance in the nAnTi-CAGE protocol
 ([Murata _et al._, 2014](http://dx.doi.org/10.1007%2F978-1-4939-0805-9_7)).
 
 
+### <a name="RNA_origin">On what RNAs does it work ?</a>
+
+_nanoCAGE_ works on total RNAs from a broad range of species.  We used it
+mostly on vertebrate RNAs, but there is also a published report using insect
+total RNA (Honey Bee, [Khamis _et al._, 2015](http://dx.doi.org/10.1038/srep11136)).
+We also produced libraries from plant (_Arabidopsis_), Yeast (_S. pombe_) and
+bacterial (_E. coli_) total RNA (unpublished results). _nanoCAGE_ also work on
+other RNA preparations, such as from ribosome pulldown ([Kratz _et al._,
+2014](http://dx.doi.org/10.1101/gr.164095.113)). 
+
+
 Bibliography
 ------------
 

Removed extra line.
diff --git a/bibliography/26073445.mdwn b/bibliography/26073445.mdwn
index 5a2e39e..66b752b 100644
--- a/bibliography/26073445.mdwn
+++ b/bibliography/26073445.mdwn
@@ -6,6 +6,5 @@ Essack M, Umylny B, Jankovic BR, Naeger NL, Suzuki M, Harbers M<sup>✉</sup>,
 Robinson GE<sup>✉</sup>, Bajic VB<sup>✉</sup>.
 _Insights into the Transcriptional Architecture of Behavioral Plasticity in the
 Honey Bee _Apis mellifera_._
-
 [[!doi 10.1038/srep11136 desc="Sci Rep. **2015** Jun 15;5:11136"]]
 [[!PMID 26073445]]

Honey bee !
diff --git a/bibliography/26073445.mdwn b/bibliography/26073445.mdwn
new file mode 100644
index 0000000..5a2e39e
--- /dev/null
+++ b/bibliography/26073445.mdwn
@@ -0,0 +1,11 @@
+[[!tag bibliography nanoCAGE]]
+[[!meta date="15 Jun 2015"]]
+
+Khamis AM<sup>☮</sup>, Hamilton AR<sup>☮</sup>, Medvedeva YA, Alam T, Alam I,
+Essack M, Umylny B, Jankovic BR, Naeger NL, Suzuki M, Harbers M<sup>✉</sup>,
+Robinson GE<sup>✉</sup>, Bajic VB<sup>✉</sup>.
+_Insights into the Transcriptional Architecture of Behavioral Plasticity in the
+Honey Bee _Apis mellifera_._
+
+[[!doi 10.1038/srep11136 desc="Sci Rep. **2015** Jun 15;5:11136"]]
+[[!PMID 26073445]]

updated PO files
diff --git a/News/2015/new_member_may.ja.po b/News/2015/new_member_may.ja.po
new file mode 100644
index 0000000..7ff2cd5
--- /dev/null
+++ b/News/2015/new_member_may.ja.po
@@ -0,0 +1,39 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-05-26 00:05+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta title=\"Welcome to Iris Bian\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"18 May 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag People/Iris_Bian]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"We are happy to welcome Iris Bian, a student of the University of Toronto, "
+"Canada, who visits us to explore the "
+"[Nanpore](https://www.nanoporetech.com/)  technology with us."
+msgstr ""

New student last year.
diff --git a/News/2015/new_member_may.mdwn b/News/2015/new_member_may.mdwn
new file mode 100644
index 0000000..d41ecbe
--- /dev/null
+++ b/News/2015/new_member_may.mdwn
@@ -0,0 +1,7 @@
+[[!meta title="Welcome to Iris Bian"]]
+[[!meta date="18 May 2015"]]
+[[!tag People/Iris_Bian]]
+
+We are happy to welcome Iris Bian, a student of the University of Toronto,
+Canada, who visits us to explore the [Nanpore](https://www.nanoporetech.com/)
+technology with us.

updated PO files
diff --git a/Openings.ja.po b/Openings.ja.po
index fa2cdec..03e92de 100644
--- a/Openings.ja.po
+++ b/Openings.ja.po
@@ -2,7 +2,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: GMTU\n"
-"POT-Creation-Date: 2015-05-06 12:00+0000\n"
+"POT-Creation-Date: 2015-05-06 12:01+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: GMTU\n"
@@ -26,8 +26,8 @@ msgstr ""
 #. type: Bullet: ' * '
 msgid ""
 "The European Molecular Biology Organisation, [EMBO](http://www.embo.org/"
-"funding-awards/fellowships/long-term-fellowships): next deadline is 13 "
-"February 2015."
+"funding-awards/fellowships/long-term-fellowships): next deadline is 10 "
+"August 2015."
 msgstr ""
 
 #. type: Bullet: ' * '

Next deadline for EMBO feilowships.
diff --git a/Openings.mdwn b/Openings.mdwn
index 845f0e8..e552912 100644
--- a/Openings.mdwn
+++ b/Openings.mdwn
@@ -7,7 +7,7 @@ one call per year.
 
  * The European Molecular Biology Organisation,
    [EMBO](http://www.embo.org/funding-awards/fellowships/long-term-fellowships):
-   next deadline is 13 February 2015.
+   next deadline is 10 August 2015.
 
  * The Japanese Society for the Promotion of Science
    ([JSPS](http://www.jsps.go.jp/english/e-fellow/postdoctoral.html#long)):

updated PO files
diff --git a/Openings.ja.po b/Openings.ja.po
index f9d4df4..fa2cdec 100644
--- a/Openings.ja.po
+++ b/Openings.ja.po
@@ -2,7 +2,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: GMTU\n"
-"POT-Creation-Date: 2014-10-01 09:47+0000\n"
+"POT-Creation-Date: 2015-05-06 12:00+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: GMTU\n"
@@ -52,8 +52,8 @@ msgstr ""
 msgid ""
 "The European Union's [Marie Skłodowska-Curie Individual Fellowships](http://"
 "ec.europa.eu/research/participants/portal/desktop/en/opportunities/h2020/"
-"topics/4052-msca-if-2014-gf.html)  (global version), previous deadline: 11 "
-"September 2014."
+"topics/10058-msca-if-2015-gf.html)  (global version), previous deadline: 10 "
+"September 2015."
 msgstr ""
 
 #. type: Bullet: ' * '

New deadline for Marie Curie fellowships.
diff --git a/Openings.mdwn b/Openings.mdwn
index c3c88fc..845f0e8 100644
--- a/Openings.mdwn
+++ b/Openings.mdwn
@@ -21,8 +21,8 @@ one call per year.
    ([HFSP](http://www.hfsp.org/funding/postdoctoral-fellowships)), next
    deadline: summer 2015.
 
- * The European Union's [Marie Skłodowska-Curie Individual Fellowships](http://ec.europa.eu/research/participants/portal/desktop/en/opportunities/h2020/topics/4052-msca-if-2014-gf.html)
-   (global version), previous deadline: 11 September 2014.
+ * The European Union's [Marie Skłodowska-Curie Individual Fellowships](http://ec.europa.eu/research/participants/portal/desktop/en/opportunities/h2020/topics/10058-msca-if-2015-gf.html)
+   (global version), previous deadline: 10 September 2015.
 
  * RIKEN Foreign Postdoctoral Researcher program ([FPR](http://www.riken.jp/en/careers/programs/fpr/)).
    Next deadline: to be announced.

updated PO files
diff --git a/News/2015/meet_us_at_11AGW.ja.po b/News/2015/meet_us_at_11AGW.ja.po
new file mode 100644
index 0000000..d7e2480
--- /dev/null
+++ b/News/2015/meet_us_at_11AGW.ja.po
@@ -0,0 +1,41 @@
+# SOME DESCRIPTIVE TITLE
+# Copyright (C) YEAR Free Software Foundation, Inc.
+# This file is distributed under the same license as the PACKAGE package.
+# FIRST AUTHOR <EMAIL@ADDRESS>, YEAR.
+#
+#, fuzzy
+msgid ""
+msgstr ""
+"Project-Id-Version: PACKAGE VERSION\n"
+"POT-Creation-Date: 2015-04-10 11:26+0000\n"
+"PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
+"Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
+"Language-Team: LANGUAGE <LL@li.org>\n"
+"Language: \n"
+"MIME-Version: 1.0\n"
+"Content-Type: text/plain; charset=UTF-8\n"
+"Content-Transfer-Encoding: 8bit\n"
+
+#. type: Plain text
+#, no-wrap
+msgid ""
+"[[!meta title=\"Meet us at The 11th International Workshop on Advanced "
+"Genomics.\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!meta date=\"10 Apr 2015\"]]\n"
+msgstr ""
+
+#. type: Plain text
+#, no-wrap
+msgid "[[!tag Meeting]]\n"
+msgstr ""
+
+#. type: Plain text
+msgid ""
+"We will be presenting multiple posters on our single-cell research at the "
+"[The 11th International Workshop on Advanced "
+"Genomics](http://gt164.jpn.org/11agw/en/)  on May 21th.  See you there !"
+msgstr ""

Meet us at 11AGW.
diff --git a/News/2015/meet_us_at_11AGW.mdwn b/News/2015/meet_us_at_11AGW.mdwn
new file mode 100644
index 0000000..7a93795
--- /dev/null
+++ b/News/2015/meet_us_at_11AGW.mdwn
@@ -0,0 +1,7 @@
+[[!meta title="Meet us at The 11th International Workshop on Advanced Genomics."]]
+[[!meta date="10 Apr 2015"]]
+[[!tag Meeting]]
+
+We will be presenting multiple posters on our single-cell research at the [The
+11th International Workshop on Advanced Genomics](http://gt164.jpn.org/11agw/en/)
+on May 21th.  See you there !

updated PO files
diff --git a/People.ja.po b/People.ja.po
index 3e16452..b1c2548 100644
--- a/People.ja.po
+++ b/People.ja.po
@@ -1,7 +1,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: GMTU\n"
-"POT-Creation-Date: 2015-01-17 12:19+0000\n"
+"POT-Creation-Date: 2015-04-07 08:15+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: GMTU\n"
@@ -20,10 +20,6 @@ msgid "[[Charles_Plessy]] (unit leader)"
 msgstr ""
 
 #. type: Bullet: '* '
-msgid "Anton Kratz (JSPS fellow)"
-msgstr ""
-
-#. type: Bullet: '* '
 msgid "Sachi Kato (technician)"
 msgstr ""
 
@@ -34,7 +30,3 @@ msgstr ""
 #. type: Bullet: '* '
 msgid "Ophélie Arnaud (post-doctoral researcher)"
 msgstr ""
-
-#. type: Bullet: '* '
-msgid "Elo Madissoon (visiting student)"
-msgstr ""

Update of the members list.
diff --git a/People.mdwn b/People.mdwn
index 693e667..8c6cfa6 100644
--- a/People.mdwn
+++ b/People.mdwn
@@ -2,8 +2,6 @@ People
 ======
 
 * [[Charles_Plessy]] (unit leader)
-* Anton Kratz (JSPS fellow)
 * Sachi Kato (technician)
 * Stéphane Poulain (research scientist)
 * Ophélie Arnaud (post-doctoral researcher)
-* Elo Madissoon (visiting student)

Bibliography for pseudo-random primers.
diff --git a/bibliography/19668204.mdwn b/bibliography/19668204.mdwn
new file mode 100644
index 0000000..165d7fc
--- /dev/null
+++ b/bibliography/19668204.mdwn
@@ -0,0 +1,7 @@
+[[!tag bibliography pseudo-random]]
+[[!meta date="01 Sep 2009"]]
+
+Armour CD, Castle JC, Chen R, Babak T, Loerch P, Jackson S, Shah JK, Dey J, Rohl CA, Johnson JM, Raymond CK.
+_Digital transcriptome profiling using selective hexamer priming for cDNA synthesis._
+[[!doi 10.1038/nmeth.1360 desc="Nat Methods. **2009** Sep;6(9):647-9."]]
+[[!PMID 19668204]]

updated PO files
diff --git a/Publications/Purkinje-2014.ja.po b/Publications/Purkinje-2014.ja.po
index f2b8aaa..7771fae 100644
--- a/Publications/Purkinje-2014.ja.po
+++ b/Publications/Purkinje-2014.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2015-01-18 06:41+0000\n"
+"POT-Creation-Date: 2015-01-18 06:48+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -62,8 +62,8 @@ msgstr ""
 #. type: Plain text
 msgid ""
 "_See also the coverage in the [Asian Scientist](http://www.asianscientist."
-"com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in BioGARAGE issue 23, "
-"page 15."
+"com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in [BioGARAGE issue 23]"
+"(http://go.lne.st/l/66312/2014-12-30/lk/66312/90/BioGARAGE_web.pdf), page 15."
 msgstr ""
 
 #. type: Plain text

Link to BioGARAGE.
diff --git a/Publications/Purkinje-2014.mdwn b/Publications/Purkinje-2014.mdwn
index 0eb6286..5bd34f8 100644
--- a/Publications/Purkinje-2014.mdwn
+++ b/Publications/Purkinje-2014.mdwn
@@ -22,7 +22,7 @@ promoters of unknown genes.  This article is the reference use for the
 tools](https://github.com/nicolas-bertin/CAGEscan-Clustering) for which we
 released the source code in 2013.
 
-_See also the coverage in the [Asian Scientist](http://www.asianscientist.com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in BioGARAGE issue 23, page 15.
+_See also the coverage in the [Asian Scientist](http://www.asianscientist.com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in [BioGARAGE issue 23](http://go.lne.st/l/66312/2014-12-30/lk/66312/90/BioGARAGE_web.pdf), page 15.
 
 <small>_Note added on August 6<sup>th</sup>: see also the analysis of
 hippocampal dendritic RNA using a similar method, published recently by [[!doi

updated PO files
diff --git a/Publications/Purkinje-2014.ja.po b/Publications/Purkinje-2014.ja.po
index 1ecb11d..f2b8aaa 100644
--- a/Publications/Purkinje-2014.ja.po
+++ b/Publications/Purkinje-2014.ja.po
@@ -7,7 +7,7 @@
 msgid ""
 msgstr ""
 "Project-Id-Version: PACKAGE VERSION\n"
-"POT-Creation-Date: 2014-08-06 01:41+0000\n"
+"POT-Creation-Date: 2015-01-18 06:41+0000\n"
 "PO-Revision-Date: YEAR-MO-DA HO:MI+ZONE\n"
 "Last-Translator: FULL NAME <EMAIL@ADDRESS>\n"
 "Language-Team: LANGUAGE <LL@li.org>\n"
@@ -62,7 +62,8 @@ msgstr ""
 #. type: Plain text
 msgid ""
 "_See also the coverage in the [Asian Scientist](http://www.asianscientist."
-"com/in-the-lab/teasing-purkinje-proteins-2014/)_."
+"com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in BioGARAGE issue 23, "
+"page 15."
 msgstr ""
 
 #. type: Plain text

Mention BioGARAGE.
diff --git a/Publications/Purkinje-2014.mdwn b/Publications/Purkinje-2014.mdwn
index 414b566..0eb6286 100644
--- a/Publications/Purkinje-2014.mdwn
+++ b/Publications/Purkinje-2014.mdwn
@@ -22,7 +22,7 @@ promoters of unknown genes.  This article is the reference use for the
 tools](https://github.com/nicolas-bertin/CAGEscan-Clustering) for which we
 released the source code in 2013.
 
-_See also the coverage in the [Asian Scientist](http://www.asianscientist.com/in-the-lab/teasing-purkinje-proteins-2014/)_.
+_See also the coverage in the [Asian Scientist](http://www.asianscientist.com/in-the-lab/teasing-purkinje-proteins-2014/)_ and in BioGARAGE issue 23, page 15.
 
 <small>_Note added on August 6<sup>th</sup>: see also the analysis of
 hippocampal dendritic RNA using a similar method, published recently by [[!doi